Molecular cloning and expression analysis of FvMYB1 from Fraxinus velutina Torr.

Tian LI; Zhen-Ying PENG; Yu-Ping BI; Zhong-Xue FAN

Article Details

Molecular cloning and expression analysis of FvMYB1 from Fraxinus velutina Torr.

Authors:

Tian LI | Zhen-Ying PENG | Yu-Ping BI | Zhong-Xue FAN

Publisher:

TUBITAK

Journal:

Turkish Journal of Agriculture and Forestry 2013 Vol.37 Issue 5, pp.517-526

Document Type:

Article

Publication Date:

4/2013

Document Language:

Turkish

Document ID (Org No.):

2000807554

Frequency:

Bimonthly

eISSN:

1303-6173

pISSN:

1300-011X

Keywords:

Abstract:

 

V-myb avian myeloblastosis viral oncogene homolog (MYB) transcription factors play important roles in the processes of plant growth, development, and stress responses. In this study, a full-length cDNA sequence of a MYB gene, designated FvMYB1, was isolated from Arizona ash (Fraxinus velutina Torr.) for the first time. Sequence analysis showed that the deduced amino acid sequence of FvMYB1 encoded novel MYB proteins with single DNA binding domains. Based on the R3 domain amino acid sequence, the FvMYB1 was closely related to some proteins whose functions were known. The expression pattern of FvMYB1 in different organs of Arizona ash was analyzed by semiquantitative RT-PCR and real-time PCR (qRT-PCR). Results showed that this gene was widely distributed in all the tested organs and the expression level of FvMYB1 was the highest in stems and the lowest in roots. The gene expression level decreased dramatically in roots under salt treatment (300 mM, 24 h), while no obvious change was observed in stems. Subcellular localization indicated that FvMYB1 was localized in the nucleus. This study will lead to further research in resistance to abiotic stress in Arizona ash.

 

V-myb avian myeloblastosis viral oncogene homolog (MYB) transcription factors play important roles in the processes of plant growth, development, and stress responses. In this study, a full-length cDNA sequence of a MYB gene, designated FvMYB1, was isolated from Arizona ash (Fraxinus velutina Torr.) for the first time. Sequence analysis showed that the deduced amino acid sequence of FvMYB1 encoded novel MYB proteins with single DNA binding domains. Based on the R3 domain amino acid sequence, the FvMYB1 was closely related to some proteins whose functions were known. The expression pattern of FvMYB1 in different organs of Arizona ash was analyzed by semiquantitative RT-PCR and real-time PCR (qRT-PCR). Results showed that this gene was widely distributed in all the tested organs and the expression level of FvMYB1 was the highest in stems and the lowest in roots. The gene expression level decreased dramatically in roots under salt treatment (300 mM, 24 h), while no obvious change was observed in stems. Subcellular localization indicated that FvMYB1 was localized in the nucleus. This study will lead to further research in resistance to abiotic stress in Arizona ash.

Article Details

Molecular cloning and expression analysis of FvMYB1 from Fraxinus velutina Torr.

Authors:

Tian LI | Zhen-Ying PENG | Yu-Ping BI | Zhong-Xue FAN

Publisher:

TUBITAK

Journal:

Turkish Journal of Agriculture and Forestry 2013 Vol.37 Issue 5, pp.517-526

Document Type:

Article

Publication Date:

4/2013

Document Language:

Turkish

Document ID (Org No.):

2000807554

Frequency:

Bimonthly

eISSN:

1303-6173

pISSN:

1300-011X

Key words: Arizona ash

expression analysis

molecular cloning

salt stress

V-myb avian myeloblastosis viral oncogene homolog

Issues

Article Details

Molecular cloning and expression analysis of FvMYB1 from Fraxinus velutina Torr.

Authors: Tian LI | Zhen-Ying PENG | Yu-Ping BI | Zhong-Xue FAN

Publisher: TUBITAK

Journal: Turkish Journal of Agriculture and Forestry 2013 Vol.37 Issue 5, pp.517-526

Document Type: Article

Publication Date: 4/2013

Document Language: Turkish

Document ID (Org No.): 2000807554

Frequency: Bimonthly

eISSN: 1303-6173

pISSN: 1300-011X

Key words: Arizona ash

expression analysis

molecular cloning

salt stress

V-myb avian myeloblastosis viral oncogene homolog

Issues

Authors:

Tian LI | Zhen-Ying PENG | Yu-Ping BI | Zhong-Xue FAN

Publisher:

TUBITAK

Journal:

Turkish Journal of Agriculture and Forestry 2013 Vol.37 Issue 5, pp.517-526

Document Type:

Article

Publication Date:

4/2013

Document Language:

Turkish

Document ID (Org No.):

2000807554

Frequency:

Bimonthly

eISSN:

1303-6173

pISSN:

1300-011X