In vitro Micropropagation of Immortelle Grass (Helichrysum italicum (ROTH) G. DON)

İbrahim Uz; Şeyda Savalan

Article Details

In vitro Micropropagation of Immortelle Grass (Helichrysum italicum (ROTH) G. DON)

Authors:

İbrahim Uz | Şeyda Savalan

Publisher:

Tekirdag Namik Kemal University

Main Topics:

Tarımsal Biyoteknoloji (Diğer)

Journal:

Tekirdağ Ziraat Fakültesi Dergisi Vol.21 Issue 1, pp.246-255

Document Type:

Article

Publication Date:

1/2024

Document Language:

English

Document ID (Org No.):

2001310413

pISSN:

1302-7050

Keywords:

Abstract:

 Immortelle grass Helichrysum italicum Roth G. Don, which spreads in the Southern Marmara and Aegean regions, can be grown in arid and semi-arid regions. In addition, due to its rich essential oil and secondary metabolite content, it has an important place in modern medicine and cosmetics, including traditional treatment methods. Although the propagation of plants by shoot regeneration in vitro has been achieved in many plant species, studies on tissue culture in immortelle grass are limited. This study aims to optimize the tissue culture study in immortelle grass and provide a basis for the next in vitro, molecular, and secondary metabolite studies. In addition, it promotes the plant by optimizing the healthy and disease-free seedling production method for cultural agriculture in the region. Three different 15, 25, and 35 NaOCl concentrations were tested for 10 and 20 minutes during the sterilization phase of the explants. The most successful result was obtained in the medium containing 35 NaOCl for 10 minutes. Sterilized explants were transferred to MS and Gamborg B5 nutrient media containing BAP, GA, and NAA plant growth regulators for shoot regeneration. The best regeneration in explants was obtained in MS medium containing 0.5 mg L-1 BAP, 1 mg L-1 GA, and 0.2 mg L-1 NAA. No growth was observed in trials containing Gamborg B5, and vitrification and darkening occurred in the explants. After four weeks, the shoots reaching a length of 3 cm were taken into MS and ½MS medium containing 0 MS, 0.5 mg L-1 IBA, 1 mg L-1 IBA, 1.5 mg L-1, and 2 mg L-1 IBA as a rooting medium. 100 rooting was observed in all prepared media within four weeks. As a result of micropropagation studies, the rooted plants were transferred to the acclimatization stage within three months and then moved to the pots in the greenhouse and to the field one month later.

 Immortelle grass Helichrysum italicum Roth G. Don, which spreads in the Southern Marmara and Aegean regions, can be grown in arid and semi-arid regions. In addition, due to its rich essential oil and secondary metabolite content, it has an important place in modern medicine and cosmetics, including traditional treatment methods. Although the propagation of plants by shoot regeneration in vitro has been achieved in many plant species, studies on tissue culture in immortelle grass are limited. This study aims to optimize the tissue culture study in immortelle grass and provide a basis for the next in vitro, molecular, and secondary metabolite studies. In addition, it promotes the plant by optimizing the healthy and disease-free seedling production method for cultural agriculture in the region. Three different 15, 25, and 35 NaOCl concentrations were tested for 10 and 20 minutes during the sterilization phase of the explants. The most successful result was obtained in the medium containing 35 NaOCl for 10 minutes. Sterilized explants were transferred to MS and Gamborg B5 nutrient media containing BAP, GA, and NAA plant growth regulators for shoot regeneration. The best regeneration in explants was obtained in MS medium containing 0.5 mg L-1 BAP, 1 mg L-1 GA, and 0.2 mg L-1 NAA. No growth was observed in trials containing Gamborg B5, and vitrification and darkening occurred in the explants. After four weeks, the shoots reaching a length of 3 cm were taken into MS and ½MS medium containing 0 MS, 0.5 mg L-1 IBA, 1 mg L-1 IBA, 1.5 mg L-1, and 2 mg L-1 IBA as a rooting medium. 100 rooting was observed in all prepared media within four weeks. As a result of micropropagation studies, the rooted plants were transferred to the acclimatization stage within three months and then moved to the pots in the greenhouse and to the field one month later.

 Immortelle grass Helichrysum italicum Roth G. Don, which spreads in the Southern Marmara and Aegean regions, can be grown in arid and semi-arid regions. In addition, due to its rich essential oil and secondary metabolite content, it has an important place in modern medicine and cosmetics, including traditional treatment methods. Although the propagation of plants by shoot regeneration in vitro has been achieved in many plant species, studies on tissue culture in immortelle grass are limited. This study aims to optimize the tissue culture study in immortelle grass and provide a basis for the next in vitro, molecular, and secondary metabolite studies. In addition, it promotes the plant by optimizing the healthy and disease-free seedling production method for cultural agriculture in the region. Three different 15, 25, and 35 NaOCl concentrations were tested for 10 and 20 minutes during the sterilization phase of the explants. The most successful result was obtained in the medium containing 35 NaOCl for 10 minutes. Sterilized explants were transferred to MS and Gamborg B5 nutrient media containing BAP, GA, and NAA plant growth regulators for shoot regeneration. The best regeneration in explants was obtained in MS medium containing 0.5 mg L-1 BAP, 1 mg L-1 GA, and 0.2 mg L-1 NAA. No growth was observed in trials containing Gamborg B5, and vitrification and darkening occurred in the explants. After four weeks, the shoots reaching a length of 3 cm were taken into MS and ½MS medium containing 0 MS, 0.5 mg L-1 IBA, 1 mg L-1 IBA, 1.5 mg L-1, and 2 mg L-1 IBA as a rooting medium. 100 rooting was observed in all prepared media within four weeks. As a result of micropropagation studies, the rooted plants were transferred to the acclimatization stage within three months and then moved to the pots in the greenhouse and to the field one month later.

 Güney Marmara ve Ege bölgesinde yayılış gösteren ölmez otu bitkisi Helichrysum italicum Roth G. Don kurak ve yarı kurak bölgelerde yetiştirilebilmektedir. Ayrıca zengin uçucu yağ ve sekonder metabolit içeriğinden dolayı geleneksel tedavi yöntemleri dahil modern tıp ve kozmetik alanında önemli bir yere sahiptir. Bitkilerin in vitro koşullarda sürgün rejenerasyonu yoluyla çoğaltılması birçok bitki türünde elde edilmiş olmasına rağmen, ölmez otunda doku kültürü üzerine yapılan çalışmalar oldukça sınırlıdır. Bu çalışmanın amacı, ölmez otu bitkisinde doku kültürü çalışmasının optimizasyonunun yapılması ve bir sonraki in vitro, moleküler ve sekonder metabolit çalışmalarına zemin sağlamaktır. Bunun yanı sıra bölgede kültür tarımı için sağlıklı ve hastalıksız fide üretimi yönteminin optimize edilerek bitkiyi tanıtmaktır. Eksplantların sterilizasyon aşamasında üç farklı 15, 25 ve 35 NaOCl konsantrasyonu 10 ve 20 dakika süre ile denenmiştir. En başarılı sonuç 10 dakika 35 NaOCl içeren ortamdan elde edilmiştir. Steril edilen eksplantlar sürgün rejenerasyonu için BAP, GA ve NAA bitki büyüme düzenleyicileri içeren MS ve Gamborg B5 besin ortamlarına aktarılmıştır. Eksplantlarda en iyi rejenerasyon 0.5 mg L-1 BAP, 1 mg L-1 GA ve 0.2 mg L-1 NAA içeren MS ortamında elde edilmiştir. Gamborg B5 içeren denemelerde herhangi gelişim gözlenememiş olup eksplantlarda vitrifikasyon ve kararma oluşmuştur. Dört hafta sonunda 3 cm uzunluğa ulaşan sürgünler köklendirme ortamı olarak 0 MS, 0.5 mg L-1 IBA, 1 mg IBA, 1.5 mg L-1 ve 2 mg L-1 IBA içeren MS ve ½MS ortamına alınmıştır. Hazırlanan tüm ortamlarda dört hafta içerisinde 100 köklenme görülmüştür. Mikroçoğaltım çalışmaları sonucu üç ay içerisinde köklenmiş bitkiler aklimatizasyon aşamasına alındıktan sonra seradaki saksılara ve bir ay sonra tarlaya aktarılmıştır.

Article Details

In vitro Micropropagation of Immortelle Grass (Helichrysum italicum (ROTH) G. DON)

Authors:

İbrahim Uz | Şeyda Savalan

Publisher:

Tekirdag Namik Kemal University

Main Topics:

Tarımsal Biyoteknoloji (Diğer)

Journal:

Tekirdağ Ziraat Fakültesi Dergisi Vol.21 Issue 1, pp.246-255

Document Type:

Article

Publication Date:

1/2024

Document Language:

English

Document ID (Org No.):

2001310413

pISSN:

1302-7050

Helichrysum italicum

Regeneration

Rooting

Acclimatization

Gamborg B5

MSHelichrysum italicum

Rejenerasyon

Köklendirme

Aklimatizasyon

Gamborg B5

MS

Issues

Article Details

Authors: İbrahim Uz | Şeyda Savalan

Publisher: Tekirdag Namik Kemal University

Main Topics: Tarımsal Biyoteknoloji (Diğer)

Journal: Tekirdağ Ziraat Fakültesi Dergisi Vol.21 Issue 1, pp.246-255

Document Type: Article

Publication Date: 1/2024

Document Language: English

Document ID (Org No.): 2001310413

pISSN: 1302-7050

Issues

Authors:

İbrahim Uz | Şeyda Savalan

Publisher:

Tekirdag Namik Kemal University

Main Topics:

Tarımsal Biyoteknoloji (Diğer)

Journal:

Tekirdağ Ziraat Fakültesi Dergisi Vol.21 Issue 1, pp.246-255

Document Type:

Article

Publication Date:

1/2024

Document Language:

English

Document ID (Org No.):

2001310413

pISSN:

1302-7050