Influence of Process Conditions on Measles Virus Stability

K. Weiss; D. Salzig; J. Gerstenberger; Y. R&ouml;der; K. Cichutek; R. Pörtner; P. Czermak; M. D. Mühlebach

doi:10.3844/ajbbsp.2013.243.254

Article Details

Influence of Process Conditions on Measles Virus Stability

Authors:

K. Weiss | D. Salzig | J. Gerstenberger | Y. Röder | K. Cichutek | R. Pörtner | P. Czermak | M. D. Mühlebach

Publisher:

Science Publications

Journal:

American Journal of Biochemistry and Biotechnology Vol.9 Issue 3, pp.243-254

Document Type:

Article

Publication Date:

9/2013

Document Language:

English

Document ID (Org No.):

2001397146

pISSN:

1558-6332

DOI:

10.3844/ajbbsp.2013.243.254

Keywords:

Abstract:

 Recombinant measles viruses are currently tested in clinical trials as oncolytic agent to be applied in cancer therapy. Contrary to their use as vaccine where 103 infectious virus particles per dose are needed, for cancer therapy 109 virus particles should be provided per dose. This leads to other challenges for the production process when compared to vaccine production. This study presents measles virus stability with regard to conditions during production and storage of the virus. Relevant process parameters such as temperature (4-37°C), pH (pH 4-11), conductivity (1.5 to 137.5 mS cm-1) and oxygen partial pressure were analyzed. The infectivity of measles virus particles decreased highly at 37 and 32°C, while at 22 and 4°C it remained stable for several hours or even days, respectively. The thermal inactivation reactions followed first order kinetics and the thermodynamic parameters enthalpy and entropy were estimated. Towards changes in pH measles virus particles were very sensitive, while no inactivation could be observed with varying conductivity. Measles virus incubation at an oxygen partial pressure of 100% did not lead to any loss of infectivity. The results show which parameters should be considered and controlled strongly in the production process to further raise measles virus yields for the high amount needed in cancer therapy approaches.

Article Details

Influence of Process Conditions on Measles Virus Stability

Authors:

K. Weiss | D. Salzig | J. Gerstenberger | Y. Röder | K. Cichutek | R. Pörtner | P. Czermak | M. D. Mühlebach

Publisher:

Science Publications

Journal:

American Journal of Biochemistry and Biotechnology Vol.9 Issue 3, pp.243-254

Document Type:

Article

Publication Date:

9/2013

Document Language:

English

Document ID (Org No.):

2001397146

pISSN:

1558-6332

DOI:

10.3844/ajbbsp.2013.243.254

Measles Virus

Virus Stability

Virus Inactivation

Oncolytic Agent

Issues

Article Details

Influence of Process Conditions on Measles Virus Stability

Authors: K. Weiss | D. Salzig | J. Gerstenberger | Y. R&ouml;der | K. Cichutek | R. Pörtner | P. Czermak | M. D. Mühlebach

Publisher: Science Publications

Journal: American Journal of Biochemistry and Biotechnology Vol.9 Issue 3, pp.243-254

Document Type: Article

Publication Date: 9/2013

Document Language: English

Document ID (Org No.): 2001397146

pISSN: 1558-6332

DOI: 10.3844/ajbbsp.2013.243.254

Measles Virus

Virus Stability

Virus Inactivation

Oncolytic Agent

Issues

Authors:

K. Weiss | D. Salzig | J. Gerstenberger | Y. Röder | K. Cichutek | R. Pörtner | P. Czermak | M. D. Mühlebach

Publisher:

Science Publications

Journal:

American Journal of Biochemistry and Biotechnology Vol.9 Issue 3, pp.243-254

Document Type:

Article

Publication Date:

9/2013

Document Language:

English

Document ID (Org No.):

2001397146

pISSN:

1558-6332

DOI:

10.3844/ajbbsp.2013.243.254