Identification of pituitary-specific transcription factor-1 (PIT-1) and  leptin gene (LEP) polymorphism of Holstein cattle reared in Turkey

Davut BAYRAM; Korhan ARSLAN; Bilal AKYÜZ; Kaan Muhsin İŞCAN

doi:https://doi.org/10.1501/Vetfak_0000002818

Article Details

Identification of pituitary-specific transcription factor-1 (PIT-1) and leptin gene (LEP) polymorphism of Holstein cattle reared in Turkey

Authors:

Davut BAYRAM | Korhan ARSLAN | Bilal AKYÜZ | Kaan Muhsin İŞCAN

Main Topics:

Animal husbandry

Journal:

Ankara Üniversitesi Veteriner Fakültesi Dergisi 2017 Vol.64 Issue 4, pp.337-343

Document Type:

Article

Publication Date:

11/2017

Document Language:

English

Document ID (Org No.):

2000849903

DOI:

https://doi.org/10.1501/Vetfak_0000002818

Keywords:

Abstract:

 

Purpose of this study was to examine leptin (LEP) and pituitary-specific transcription factor-1 (Pit-I) gene polymorphisms in Holstein cattle (n=352) in Turkey. In order to determine the Pit-I-HinfI and LEP-Sau3AI polymorphisms, polymerase chain reaction and restriction fragment length polymorphism (PCR-RFLP) were performed. A 600 bp length fragment of Pit-I and a 422 bp length fragment of LEP were amplified. In this study, two types of alleles, A and B, for both of the Pit-I and LEP genes were observed. The highest frequencies of the alleles were estimated, and were the B allele (0.68) for the Pit-I gene and the A allele (0.87) for the LEP gene. According to the results of the chi-square test, a significant deviation from the Hardy-Weinberg equilibrium was determined for both of these genes in the investigated breed (in the same order; X2:40.75, p<0.001and X2:8.4, p<0.01).

 

Bu çalışmada Türkiye’de Kayseri civarında yetiştirilen Holştayn ırkı sığırlarda (n=352) leptin (LEP) ve hipofiz bezine özgü transkripsiyon faktörü -1 (Pit-I) genlerinin polimorfizmlerinin belirlenmesi amaçlanmıştır. Pit-I-HinfI ve LEP-Sau3AI polimorfizmlerinin belirlenmesi amacı ile polimeraz zincir reaksiyonu (PZR) ve restriksiyon parça uzunluk polimorfizm (RFLP) analizi yapılmıştır. Pit-1 geni için 600 bç’lik ve LEP geni için 422 bç’lik uzunluk amplifiye edilmiştir. Çalışmada, Pit-1 ve LEP geni allellerinin her ikisinde de A ve B allelleri olmak üzere iki iki tip allel gözlendi. En yüksek frekans Pit-1 geni için A allelinde (0.87), LEP geni için ise B allelinde (0.68) olarak belirlendi. Ki-kare test sonuçlarına gore, incelenen ırkta, her iki gende de Hardy-Weinberg dengesinden anlamlı bir sapma gözlendi (aynı sıra ile; X2:40.75, p<0.001ve X2:8.4, p<0.01).

Article Details

Identification of pituitary-specific transcription factor-1 (PIT-1) and leptin gene (LEP) polymorphism of Holstein cattle reared in Turkey

Authors:

Davut BAYRAM | Korhan ARSLAN | Bilal AKYÜZ | Kaan Muhsin İŞCAN

Main Topics:

Animal husbandry

Journal:

Ankara Üniversitesi Veteriner Fakültesi Dergisi 2017 Vol.64 Issue 4, pp.337-343

Document Type:

Article

Publication Date:

11/2017

Document Language:

English

Document ID (Org No.):

2000849903

DOI:

https://doi.org/10.1501/Vetfak_0000002818

Holştayn

leptin

pit-1

polimorfizm

RFLP

Issues

Article Details

Identification of pituitary-specific transcription factor-1 (PIT-1) and leptin gene (LEP) polymorphism of Holstein cattle reared in Turkey

Authors: Davut BAYRAM | Korhan ARSLAN | Bilal AKYÜZ | Kaan Muhsin İŞCAN

Main Topics: Animal husbandry

Journal: Ankara Üniversitesi Veteriner Fakültesi Dergisi 2017 Vol.64 Issue 4, pp.337-343

Document Type: Article

Publication Date: 11/2017

Document Language: English

Document ID (Org No.): 2000849903

DOI: https://doi.org/10.1501/Vetfak_0000002818

Holştayn

leptin

pit-1

polimorfizm

RFLP

Issues

Authors:

Davut BAYRAM | Korhan ARSLAN | Bilal AKYÜZ | Kaan Muhsin İŞCAN

Main Topics:

Animal husbandry

Journal:

Ankara Üniversitesi Veteriner Fakültesi Dergisi 2017 Vol.64 Issue 4, pp.337-343

Document Type:

Article

Publication Date:

11/2017

Document Language:

English

Document ID (Org No.):

2000849903

DOI:

https://doi.org/10.1501/Vetfak_0000002818