Antibacterial Activity of Seed Extracts of Commercial and Wild Lathyrus Species

Noor Afshan KHAN; Sadaf QUERESHI; Akhilesh PANDEY; Ashutosh SRIVASTAVA

Article Details

Antibacterial Activity of Seed Extracts of Commercial and Wild Lathyrus Species

Authors:

Noor Afshan KHAN | Sadaf QUERESHI | Akhilesh PANDEY | Ashutosh SRIVASTAVA

Publisher:

TUBITAK

Journal:

Turkish Journal of Biology 2009 Vol.33 Issue 2, pp.165-169

Document Type:

Article

Publication Date:

1/2009

Document Language:

Turkish

Document ID (Org No.):

2000813228

Frequency:

Bimonthly

eISSN:

1303-6092

pISSN:

1300-0152

Keywords:

Abstract:

 

Butanolic extract of seeds from 2 different Lathyrus species (Lathyrus ratan [commercial variety] and L. aphaca [wild variety]), were investigated for their in vitro antibacterial activity. The antibacterial activity against plant and human pathogenic bacteria was evaluated on the basis of inhibition zones (IZs) measured by disc diffusion method. The maximum inhibition was shown by L. ratan against the gram-positive bacterium Staphylococcus aureus. L. aphaca seeds also showed inhibition but were inactive against Klebsiella pneumoniae. L. ratan extract was more active than L. aphaca. The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) values for both the seeds (in mg/ml) were also determined. The lowest value was obtained for Staphylococcus aureus [MIC-76.25 (L.a.) 78.5 (L.r.) MBC- 112.6 (L.a.) 98.35 (L.r.)]; thus this bacterium was most inhibited by the seed extract, whereas Bacillus subtilis was least inhibited, as indicated by MIC [(98.50 (L.a.) 96.2 (L.r.)] and MBC [169.50 (L.a.) 151.2 (L.r.)] values.

 

Butanolic extract of seeds from 2 different Lathyrus species (Lathyrus ratan [commercial variety] and L. aphaca [wild variety]), were investigated for their in vitro antibacterial activity. The antibacterial activity against plant and human pathogenic bacteria was evaluated on the basis of inhibition zones (IZs) measured by disc diffusion method. The maximum inhibition was shown by L. ratan against the gram-positive bacterium Staphylococcus aureus. L. aphaca seeds also showed inhibition but were inactive against Klebsiella pneumoniae. L. ratan extract was more active than L. aphaca. The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) values for both the seeds (in mg/ml) were also determined. The lowest value was obtained for Staphylococcus aureus [MIC-76.25 (L.a.) 78.5 (L.r.) MBC- 112.6 (L.a.) 98.35 (L.r.)]; thus this bacterium was most inhibited by the seed extract, whereas Bacillus subtilis was least inhibited, as indicated by MIC [(98.50 (L.a.) 96.2 (L.r.)] and MBC [169.50 (L.a.) 151.2 (L.r.)] values.

Article Details

Antibacterial Activity of Seed Extracts of Commercial and Wild Lathyrus Species

Authors:

Noor Afshan KHAN | Sadaf QUERESHI | Akhilesh PANDEY | Ashutosh SRIVASTAVA

Publisher:

TUBITAK

Journal:

Turkish Journal of Biology 2009 Vol.33 Issue 2, pp.165-169

Document Type:

Article

Publication Date:

1/2009

Document Language:

Turkish

Document ID (Org No.):

2000813228

Frequency:

Bimonthly

eISSN:

1303-6092

pISSN:

1300-0152

Antibacterial

Lathyrus ratan

Lathyrus aphaca

MIC

MBC

Issues

Article Details

Antibacterial Activity of Seed Extracts of Commercial and Wild Lathyrus Species

Authors: Noor Afshan KHAN | Sadaf QUERESHI | Akhilesh PANDEY | Ashutosh SRIVASTAVA

Publisher: TUBITAK

Journal: Turkish Journal of Biology 2009 Vol.33 Issue 2, pp.165-169

Document Type: Article

Publication Date: 1/2009

Document Language: Turkish

Document ID (Org No.): 2000813228

Frequency: Bimonthly

eISSN: 1303-6092

pISSN: 1300-0152

Antibacterial

Lathyrus ratan

Lathyrus aphaca

MIC

MBC

Issues

Authors:

Noor Afshan KHAN | Sadaf QUERESHI | Akhilesh PANDEY | Ashutosh SRIVASTAVA

Publisher:

TUBITAK

Journal:

Turkish Journal of Biology 2009 Vol.33 Issue 2, pp.165-169

Document Type:

Article

Publication Date:

1/2009

Document Language:

Turkish

Document ID (Org No.):

2000813228

Frequency:

Bimonthly

eISSN:

1303-6092

pISSN:

1300-0152