Molecular identification of ascochyta blight of Cicer montbretii Jaub. & Spach

Fevzi Bulat; Duygu Sarı; Hatice Sarı; Tuba Eker; Hilal Özay; Cengiz Toker

Article Details

Molecular identification of ascochyta blight of Cicer montbretii Jaub. & Spach

Authors:

Fevzi Bulat | Duygu Sarı | Hatice Sarı | Tuba Eker | Hilal Özay | Cengiz Toker

Publisher:

Akdeniz University

Main Topics:

Agricultural Engineering

Journal:

Mediterranean Agricultural Sciences Vol.36 Issue 1, pp.7-12

Document Type:

Article

Publication Date:

1/2023

Document Language:

English

Document ID (Org No.):

2001287241

pISSN:

2528-9675

Keywords:

Abstract:

 Molecular detection of ascochyta blight caused by Ascochyta rabiei Pass. Labr. is important for effective control of the ascochyta blight and efficient chickpea Cicer arietinum L. breeding program. The present research was therefore aimed to diagnose ascochyta blight of C. montbretii Jaub.  Spach via molecular techniques. Infected plant samples were collected and placed on potato dextrose agar PDA medium for 1 week at 20-24, and colonies with typical ascochyta blight symptoms were transferred to new PDA medium and incubated for 1 week at 25. DNA was isolated from small parts of fungus isolates via the CTAB method. Internal transcribed spacer ITS regions ITS-1, 5.8S rDNA subunit, ITS-2 were amplified with ITS 5 and ITS 4 primers for molecular characterization. Based on the BLAST analysis, the sequence had 99 and 100 nucleotide identity with the corresponding sequence of A. rabiei in GeneBank. To our knowledge, this is the first report of ascochyta blight of C. montbretii in Turkiye. The pathogen is considered to be co-evolved with C. montbretii. Molecular techniques, as in the present study, can be diagnosed with great accuracy, in a short time, and with relatively little effort and expense.

 Molecular detection of ascochyta blight caused by Ascochyta rabiei Pass. Labr. is important for effective control of the ascochyta blight and efficient chickpea Cicer arietinum L. breeding program. The present research was therefore aimed to diagnose ascochyta blight of C. montbretii Jaub.  Spach via molecular techniques. Infected plant samples were collected and placed on potato dextrose agar PDA medium for 1 week at 20-24, and colonies with typical ascochyta blight symptoms were transferred to new PDA medium and incubated for 1 week at 25. DNA was isolated from small parts of fungus isolates via the CTAB method. Internal transcribed spacer ITS regions ITS-1, 5.8S rDNA subunit, ITS-2 were amplified with ITS 5 and ITS 4 primers for molecular characterization. Based on the BLAST analysis, the sequence had 99 and 100 nucleotide identity with the corresponding sequence of A. rabiei in GeneBank. To our knowledge, this is the first report of ascochyta blight of C. montbretii in Turkiye. The pathogen is considered to be co-evolved with C. montbretii. Molecular techniques, as in the present study, can be diagnosed with great accuracy, in a short time, and with relatively little effort and expense.

 Molecular detection of ascochyta blight caused by Ascochyta rabiei Pass. Labr. is important for effective control of the ascochyta blight and efficient chickpea Cicer arietinum L. breeding program. The present research was therefore aimed to diagnose ascochyta blight of C. montbretii Jaub.  Spach via molecular techniques. Infected plant samples were collected and placed on potato dextrose agar PDA medium for 1 week at 20-24, and colonies with typical ascochyta blight symptoms were transferred to new PDA medium and incubated for 1 week at 25. DNA was isolated from small parts of fungus isolates via the CTAB method. Internal transcribed spacer ITS regions ITS-1, 5.8S rDNA subunit, ITS-2 were amplified with ITS 5 and ITS 4 primers for molecular characterization. Based on the BLAST analysis, the sequence had 99 and 100 nucleotide identity with the corresponding sequence of A. rabiei in GeneBank. To our knowledge, this is the first report of ascochyta blight of C. montbretii in Turkiye. The pathogen is considered to be co-evolved with C. montbretii. Molecular techniques, as in the present study, can be diagnosed with great accuracy, in a short time, and with relatively little effort and expense.

 Molecular detection of ascochyta blight caused by Ascochyta rabiei Pass. Labr. is important for effective control of the ascochyta blight and efficient chickpea Cicer arietinum L. breeding program. The present research was therefore aimed to diagnose ascochyta blight of C. montbretii Jaub.  Spach via molecular techniques. Infected plant samples were collected and placed on potato dextrose agar PDA medium for 1 week at 20-24, and colonies with typical ascochyta blight symptoms were transferred to new PDA medium and incubated for 1 week at 25. DNA was isolated from small parts of fungus isolates via the CTAB method. Internal transcribed spacer ITS regions ITS-1, 5.8S rDNA subunit, ITS-2 were amplified with ITS 5 and ITS 4 primers for molecular characterization. Based on the BLAST analysis, the sequence had 99 and 100 nucleotide identity with the corresponding sequence of A. rabiei in GeneBank. To our knowledge, this is the first report of ascochyta blight of C. montbretii in Turkiye. The pathogen is considered to be co-evolved with C. montbretii. Molecular techniques, as in the present study, can be diagnosed with great accuracy, in a short time, and with relatively little effort and expense.

Article Details

Molecular identification of ascochyta blight of Cicer montbretii Jaub. & Spach

Authors:

Fevzi Bulat | Duygu Sarı | Hatice Sarı | Tuba Eker | Hilal Özay | Cengiz Toker

Publisher:

Akdeniz University

Main Topics:

Agricultural Engineering

Journal:

Mediterranean Agricultural Sciences Vol.36 Issue 1, pp.7-12

Document Type:

Article

Publication Date:

1/2023

Document Language:

English

Document ID (Org No.):

2001287241

pISSN:

2528-9675

Ascochyta blight

Ascochyta rabiei

Chickpea

Cicer montbretii

ITS

Ascochyta blight

Ascochyta rabiei

Chickpea

Cicer montbretii

ITS

Issues

Article Details

Authors: Fevzi Bulat | Duygu Sarı | Hatice Sarı | Tuba Eker | Hilal Özay | Cengiz Toker

Publisher: Akdeniz University

Main Topics: Agricultural Engineering

Journal: Mediterranean Agricultural Sciences Vol.36 Issue 1, pp.7-12

Document Type: Article

Publication Date: 1/2023

Document Language: English

Document ID (Org No.): 2001287241

pISSN: 2528-9675

Issues

Authors:

Fevzi Bulat | Duygu Sarı | Hatice Sarı | Tuba Eker | Hilal Özay | Cengiz Toker

Publisher:

Akdeniz University

Main Topics:

Agricultural Engineering

Journal:

Mediterranean Agricultural Sciences Vol.36 Issue 1, pp.7-12

Document Type:

Article

Publication Date:

1/2023

Document Language:

English

Document ID (Org No.):

2001287241

pISSN:

2528-9675