Effect of Alpha Lipoic Acid on Polychlorinated Biphenyl (Aroclor 1254)-Induced Nephrotoxicity in Rats

Mustafa Yıldız

Article Details

Effect of Alpha Lipoic Acid on Polychlorinated Biphenyl (Aroclor 1254)-Induced Nephrotoxicity in Rats

Authors:

Mustafa Yıldız

Publisher:

Afyon Kocatepe University

Main Topics:

Veteriner Histoloji ve Embriyolojisi

Journal:

Kocatepe Veteriner Dergisi Vol.16 Issue 4, pp.504-512

Document Type:

Article

Publication Date:

12/2023

Document Language:

English

Document ID (Org No.):

2001300384

pISSN:

1308-1594

Keywords:

Abstract:

 The present study aimed to determine the morphological changes in rat kidneys exposed to PCB Aroclor 1254 and to reveal the effects of ALA on those changes. Rats were randomly divided into four groups: Control, ALA, PCB and ALAPCB. The applications of ALA and PCB continued for thirty days. At the end of the experiment, the tissue samples were taken from the animals. Crossmons triple staining method was applied to kidney sections for histopathological and histomorphometric examinations. In addition, the periodic acid-Schiff PAS reaction was examined in tissue sections. Histopathologically, it was determined that glomerular degeneration and connective tissue densities in the kidney significantly increased in PCB group compared to Control group. Besides, it was found that glomerular degeneration intensity significantly decreased in ALAPCB group compared to PCB group. It was detected that the diameters of the renal corpuscle, proximal tubule, ascending limb of Henles loop and collecting duct in the kidney significantly decreased in PCB group compared to Control group. However, these parameters significantly increased in ALAPCB group compared to PCB group. While the density of glomerular mesangial matrix and glycogen density in proximal tubule epithelium significantly increased in PCB group compared to Control group, these parameters significantly decreased in ALAPCB group compared to PCB group. As a result, PCB may adversely affect renal functions by causing disorders in kidney histology. In addition, the use of ALA against PCB exposure may contribute to the preservation of kidney tissue.

 The present study aimed to determine the morphological changes in rat kidneys exposed to PCB Aroclor 1254 and to reveal the effects of ALA on those changes. Rats were randomly divided into four groups: Control, ALA, PCB and ALAPCB. The applications of ALA and PCB continued for thirty days. At the end of the experiment, the tissue samples were taken from the animals. Crossmons triple staining method was applied to kidney sections for histopathological and histomorphometric examinations. In addition, the periodic acid-Schiff PAS reaction was examined in tissue sections. Histopathologically, it was determined that glomerular degeneration and connective tissue densities in the kidney significantly increased in PCB group compared to Control group. Besides, it was found that glomerular degeneration intensity significantly decreased in ALAPCB group compared to PCB group. It was detected that the diameters of the renal corpuscle, proximal tubule, ascending limb of Henles loop and collecting duct in the kidney significantly decreased in PCB group compared to Control group. However, these parameters significantly increased in ALAPCB group compared to PCB group. While the density of glomerular mesangial matrix and glycogen density in proximal tubule epithelium significantly increased in PCB group compared to Control group, these parameters significantly decreased in ALAPCB group compared to PCB group. As a result, PCB may adversely affect renal functions by causing disorders in kidney histology. In addition, the use of ALA against PCB exposure may contribute to the preservation of kidney tissue.

 The present study aimed to determine the morphological changes in rat kidneys exposed to PCB Aroclor 1254 and to reveal the effects of ALA on those changes. Rats were randomly divided into four groups: Control, ALA, PCB and ALAPCB. The applications of ALA and PCB continued for thirty days. At the end of the experiment, the tissue samples were taken from the animals. Crossmons triple staining method was applied to kidney sections for histopathological and histomorphometric examinations. In addition, the periodic acid-Schiff PAS reaction was examined in tissue sections. Histopathologically, it was determined that glomerular degeneration and connective tissue densities in the kidney significantly increased in PCB group compared to Control group. Besides, it was found that glomerular degeneration intensity significantly decreased in ALAPCB group compared to PCB group. It was detected that the diameters of the renal corpuscle, proximal tubule, ascending limb of Henles loop and collecting duct in the kidney significantly decreased in PCB group compared to Control group. However, these parameters significantly increased in ALAPCB group compared to PCB group. While the density of glomerular mesangial matrix and glycogen density in proximal tubule epithelium significantly increased in PCB group compared to Control group, these parameters significantly decreased in ALAPCB group compared to PCB group. As a result, PCB may adversely affect renal functions by causing disorders in kidney histology. In addition, the use of ALA against PCB exposure may contribute to the preservation of kidney tissue.

 Sunulan çalışma, PCB Aroklor 1254ye maruz bırakılan sıçanların böbreklerindeki morfolojik değişikliklerin belirlenmesini ve bu değişiklikler üzerine ALAnın etkilerinin ortaya çıkarılmasını amaçladı. Sıçanlar kontrol, ALA, PCB ve ALAPCB olmak üzere rastgele dört gruba ayrıldı. ALA ve PCB uygulamaları otuz gün boyunca devam etti. Deney sonunda hayvanlardan doku örnekleri alındı. Histopatolojik ve histomorfometrik incelemeler için böbrek kesitlerine Crossmonun üçlü boyama yöntemi uygulandı. Ayrıca doku kesitlerinde periyodik asit-Schiff PAS reaksiyonu incelendi. Histopatolojik açıdan, böbrekte glomerüler dejenerasyon ile bağ doku yoğunluklarının kontrol grubuna göre PCB grubunda anlamlı bir şekilde arttığı tespit edildi. Bununla birlikte, glomerüler dejenerasyon yoğunluğunun PCB grubuna göre ALAPCB grubunda anlamlı bir şekilde azaldığı saptandı. Böbrekte renal korpüskül, proksimal tübül, çıkan henle ve toplayıcı kanal çaplarının kontrol grubuna göre PCB grubunda anlamlı bir şekilde azaldığı belirlendi. Ancak bu parametreler PCB grubuna göre ALAPCB grubunda anlamlı bir şekilde arttı. Böbrekte glomerüler mezengial matriks yoğunluğu ile proksimal tübül epitelindeki glikojen yoğunluğu kontrol grubuna göre PCB grubunda anlamlı bir şekilde artarken, bu parametreler PCB grubuna göre ALAPCB grubunda anlamlı bir şekilde azaldı. Sonuç olarak, PCB böbrek histolojisinde bozukluklara neden olarak renal fonksiyonları olumsuz etkileyebilir. Bununla birlikte, PCB maruziyetine karşı ALA kullanımı böbrek dokusunun korunmasına katkı sağlayabilir.

Article Details

Effect of Alpha Lipoic Acid on Polychlorinated Biphenyl (Aroclor 1254)-Induced Nephrotoxicity in Rats

Authors:

Mustafa Yıldız

Publisher:

Afyon Kocatepe University

Main Topics:

Veteriner Histoloji ve Embriyolojisi

Journal:

Kocatepe Veteriner Dergisi Vol.16 Issue 4, pp.504-512

Document Type:

Article

Publication Date:

12/2023

Document Language:

English

Document ID (Org No.):

2001300384

pISSN:

1308-1594

Alpha lipoic acid

Aroclor 1254

Histology

Kidney

RatAlfa lipoik asit

Aroklor 1254

Böbrek

Histoloji

Sıçan

Issues

Article Details

Authors: Mustafa Yıldız

Publisher: Afyon Kocatepe University

Main Topics: Veteriner Histoloji ve Embriyolojisi

Journal: Kocatepe Veteriner Dergisi Vol.16 Issue 4, pp.504-512

Document Type: Article

Publication Date: 12/2023

Document Language: English

Document ID (Org No.): 2001300384

pISSN: 1308-1594

Issues

Authors:

Mustafa Yıldız

Publisher:

Afyon Kocatepe University

Main Topics:

Veteriner Histoloji ve Embriyolojisi

Journal:

Kocatepe Veteriner Dergisi Vol.16 Issue 4, pp.504-512

Document Type:

Article

Publication Date:

12/2023

Document Language:

English

Document ID (Org No.):

2001300384

pISSN:

1308-1594