HLA DQA1'İN PCR ÜRÜNLERİNİN KAPİLLER JEL ELEKTROFOREZİ

Sevgi YILMAZ; Salih CENGİZ

Article Details

HLA DQA1'İN PCR ÜRÜNLERİNİN KAPİLLER JEL ELEKTROFOREZİ

Authors:

Sevgi YILMAZ | Salih CENGİZ

Publisher:

İstanbul University-Cerrahpasa

Journal:

Cerrahpaşa Tıp Dergisi 1999 Vol.30 Issue 3

Document Type:

Article

Publication Date:

8/2014

Document Language:

Turkish

Document ID (Org No.):

2000766960

Frequency:

Quarterly

pISSN:

1300-5227

Keywords:

-

Abstract:

 


Background.- HLA DQA 1 loci are highly informative polymorphic loci that are gaining popularity for identity testing. HLA DQA 1 system easy to work with, fast and reliable. This loci with PCR technique is a very promising tool for genetic investigations in both paternity and crime cases.Design.- Human genomic DNA was extracted by Chelex(r) 100 procedure. The amplification of HLA DQA 1 locus was performed by single locus PCR reaction in the case of HLA DQA 1 locus according to the manufacturer's recommendations using the Perkin Elmer AmpliType DQA 1 PCR Amplification Typing Kits. The electrophoresis of PCR products of HLA DQA 1 are carried out on agarose gel.Results.- It was shown from the obtained bands that the DNA is amplified. Hybridization is made from the PCR products by Perkin Elmer AmpliType DQA 1 PCR Amplification Typing Kits and that typed as 2,3 as a result and this allele was compared with the Capillary electrophoreograms.Conclusion.- In this study, Capillary gel electrophoresis (CGE) was performed on PCR products of HLA DQA 1 due to its advantages are ultraresolution, ultralow sample \Olume, extremely high efficiency, rapid separation time, easy quantitation and amenability to automation.

* Anahtar Kelimeler: CE, HLA DQA1

* Key Words: CE, HLA DQA1

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Article Details

HLA DQA1'İN PCR ÜRÜNLERİNİN KAPİLLER JEL ELEKTROFOREZİ

Authors: Sevgi YILMAZ | Salih CENGİZ

Publisher: İstanbul University-Cerrahpasa

Journal: Cerrahpaşa Tıp Dergisi 1999 Vol.30 Issue 3

Document Type: Article

Publication Date: 8/2014

Document Language: Turkish

Document ID (Org No.): 2000766960

Frequency: Quarterly

pISSN: 1300-5227

-

Issues

Authors:

Sevgi YILMAZ | Salih CENGİZ

Publisher:

İstanbul University-Cerrahpasa

Journal:

Cerrahpaşa Tıp Dergisi 1999 Vol.30 Issue 3

Document Type:

Article

Publication Date:

8/2014

Document Language:

Turkish

Document ID (Org No.):

2000766960

Frequency:

Quarterly

pISSN:

1300-5227