Capsaicin Shows Species and Strain-specific Activity: Investigation of the Antibacterial Effects on the Oral Pathogen Streptococcus mutans and the Oral Probiotics Streptococcus salivarius M18 and K12

Kader Doğan; Sinem Tunçer

Article Details

Capsaicin Shows Species and Strain-specific Activity: Investigation of the Antibacterial Effects on the Oral Pathogen Streptococcus mutans and the Oral Probiotics Streptococcus salivarius M18 and K12

Authors:

Kader Doğan | Sinem Tunçer

Publisher:

Hacettepe University

Main Topics:

Biochemistry and Cell Biology (Other)

Journal:

Hacettepe Journal of Biology and Chemistry Vol.52 Issue 1, pp.11-19

Document Type:

Article

Publication Date:

4/2024

Document Language:

English

Document ID (Org No.):

2001303695

pISSN:

2687-475X

Keywords:

Abstract:

 Extensive research has investigated capsaicin CAP, the primary bioactive compound in chili peppers, to explore its diverse pharmacological and physiological properties. Recently, the focus has shifted to understanding the potential effects of CAP on gut microbiota due to the strong link between gut bacterial profile and diet. However, there has been no research on the effects of CAP on oral microbiota. Therefore, our study aimed to explore the antibacterial effects of CAP on two oral probiotics, Streptococcus salivarius M18, and S. salivarius K12, along with the oral pathogen S. mutans. Previously, the anti-cancer activity of CAP had been demonstrated, and in accordance with these findings, here, we show its growth inhibitory activity on colorectal cancer cell lines. However, this study is the first to examine the impact of CAP on specific oral microorganisms while considering the oral consumption of CAP and the interconnectedness of the oral and gut microbiomes. The findings revealed that CAP exhibited antibacterial properties against the M18 strain at concentrations exceeding 100 µgmL. Surprisingly, it did not show any growth-inhibitory effects on S. salivarius K12, even at a concentration of 2 mgmL. Similarly, CAP did not inhibit the growth of S. mutans, a significant factor in dental caries. These results suggest that CAPs effects are species and strain-specific, indicating potential changes in the oral microbiota upon CAP consumption.

 Extensive research has investigated capsaicin CAP, the primary bioactive compound in chili peppers, to explore its diverse pharmacological and physiological properties. Recently, the focus has shifted to understanding the potential effects of CAP on gut microbiota due to the strong link between gut bacterial profile and diet. However, there has been no research on the effects of CAP on oral microbiota. Therefore, our study aimed to explore the antibacterial effects of CAP on two oral probiotics, Streptococcus salivarius M18, and S. salivarius K12, along with the oral pathogen S. mutans. Previously, the anti-cancer activity of CAP had been demonstrated, and in accordance with these findings, here, we show its growth inhibitory activity on colorectal cancer cell lines. However, this study is the first to examine the impact of CAP on specific oral microorganisms while considering the oral consumption of CAP and the interconnectedness of the oral and gut microbiomes. The findings revealed that CAP exhibited antibacterial properties against the M18 strain at concentrations exceeding 100 µgmL. Surprisingly, it did not show any growth-inhibitory effects on S. salivarius K12, even at a concentration of 2 mgmL. Similarly, CAP did not inhibit the growth of S. mutans, a significant factor in dental caries. These results suggest that CAPs effects are species and strain-specific, indicating potential changes in the oral microbiota upon CAP consumption.

 Extensive research has investigated capsaicin CAP, the primary bioactive compound in chili peppers, to explore its diverse pharmacological and physiological properties. Recently, the focus has shifted to understanding the potential effects of CAP on gut microbiota due to the strong link between gut bacterial profile and diet. However, there has been no research on the effects of CAP on oral microbiota. Therefore, our study aimed to explore the antibacterial effects of CAP on two oral probiotics, Streptococcus salivarius M18, and S. salivarius K12, along with the oral pathogen S. mutans. Previously, the anti-cancer activity of CAP had been demonstrated, and in accordance with these findings, here, we show its growth inhibitory activity on colorectal cancer cell lines. However, this study is the first to examine the impact of CAP on specific oral microorganisms while considering the oral consumption of CAP and the interconnectedness of the oral and gut microbiomes. The findings revealed that CAP exhibited antibacterial properties against the M18 strain at concentrations exceeding 100 µgmL. Surprisingly, it did not show any growth-inhibitory effects on S. salivarius K12, even at a concentration of 2 mgmL. Similarly, CAP did not inhibit the growth of S. mutans, a significant factor in dental caries. These results suggest that CAPs effects are species and strain-specific, indicating potential changes in the oral microbiota upon CAP consumption.

 Acı biberin ana aktif maddesi olan Kapsaisin CAP, bugüne kadar çoklu farmakolojik ve fizyolojik özellikleri açısından araştırılmıştır. Bağırsak bakteri profilinin beslenme ile güçlü bir ilişkisi olduğundan, CAPin bağırsak mikrobiyotası üzerinde etkileri giderek daha fazla ilgi çekmektedir. CAPin anti-kanser etkileri daha once çalışılmış olup, bu makalede de önceki verilerle uyumlu olarak CAPin kolorektal kanser hücrelerinde büyüme inhibe edici etkisi olduğu gösterilmektedir. Öte yandan, CAPin ağız mikrobiyotası üzerindeki etkilerini inceleyen mevcut herhangi bir araştırma bulunmamaktadır. CAPin oral tüketimi ve oral ve bağırsak mikrobiyomları arasındaki karşılıklı etkileşim göz önüne alınarak, burada, literatürde ilk kez olarak CAPinin, iki oral probiyotik olan Streptococcus salivarius M18 ve S. salivarius K12 ve oral patojen S. mutans üzerindeki antibakteriyel etkisini araştırmayı amaçladık. Sonuçlar, CAPin M18 suşu üzerinde antibakteriyel etkiye sahip olmasına rağmen konsantrasyon 100 µgmLden yüksek, 2 mgmL olarak uygulandığında dahi S. salivarius K12 üzerinde herhangi bir büyüme inhibe edici etki göstermediğini ortaya koymuştur. Benzer şekilde CAP muamelesi, diş çürüklerinde önemli bir etiyolojik faktör olan S. mutansın büyümesini de engellememiştir. Bu veriler, CAPin tür ve suşa özgü aktivite gösterdiğini ortaya koymakta olup, ayrıca CAP tüketimi sonucu ağız mikrobiyotasında olası değişikliklere dikkat çekmektedir.

Article Details

Capsaicin Shows Species and Strain-specific Activity: Investigation of the Antibacterial Effects on the Oral Pathogen Streptococcus mutans and the Oral Probiotics Streptococcus salivarius M18 and K12

Authors:

Kader Doğan | Sinem Tunçer

Publisher:

Hacettepe University

Main Topics:

Biochemistry and Cell Biology (Other)

Journal:

Hacettepe Journal of Biology and Chemistry Vol.52 Issue 1, pp.11-19

Document Type:

Article

Publication Date:

4/2024

Document Language:

English

Document ID (Org No.):

2001303695

pISSN:

2687-475X

Capsaicin

Streptococcus salivarius M18

Streptococcus salivarius K12

Streptococcus mutans

antibacterial effect

colorectal cancerKapsaisin

Streptococcus salivarius M18

Streptococcus salivarius K12

Streptococcus mutans

antibakteriyel etki

kolorektal kanser

Issues

Article Details

Authors: Kader Doğan | Sinem Tunçer

Publisher: Hacettepe University

Main Topics: Biochemistry and Cell Biology (Other)

Journal: Hacettepe Journal of Biology and Chemistry Vol.52 Issue 1, pp.11-19

Document Type: Article

Publication Date: 4/2024

Document Language: English

Document ID (Org No.): 2001303695

pISSN: 2687-475X

Issues

Authors:

Kader Doğan | Sinem Tunçer

Publisher:

Hacettepe University

Main Topics:

Biochemistry and Cell Biology (Other)

Journal:

Hacettepe Journal of Biology and Chemistry Vol.52 Issue 1, pp.11-19

Document Type:

Article

Publication Date:

4/2024

Document Language:

English

Document ID (Org No.):

2001303695

pISSN:

2687-475X