Prevalence of E. coli O157:H7 in Dairy Cattle and the Farm Environment

Esin BİRDAL; Seyyal AK

doi:https://doi.org/10.35229/jaes.387063

Article Details

Prevalence of E. coli O157:H7 in Dairy Cattle and the Farm Environment

Authors:

Esin BİRDAL | Seyyal AK

Publisher:

Bülent VEREP

Journal:

Journal of Anatolian Environmental and Animal Sciences 2018 Vol.3 Issue 2, pp.85-90

Document Type:

Article

Publication Date:

8/2018

Document Language:

English

Document ID (Org No.):

2000491147

Frequency:

Quarterly

eISSN:

2548-0006

DOI:

https://doi.org/10.35229/jaes.387063

Abstract:

 


The aims of this study were, to detect the presence,
and investigate the seasonal prevalence of E.
coli O157:H7 from feces and milk of the healthy dairy cattle in various age
groups, and from feces of the other animals (dog, cat etc.) present in the
farms. Also environmental samples from the farms which belonged to public or
private establishments, in the Marmara Region of Turkey were included. 

            For
that purpose, 96 fecal and milk samples from the same animals (except death and
slaughtering) and 96 environmental samples were collected from the determined
10 farms, in fall and spring periods.  

            In total, of 576 (288 samples in the fall period while
288 samples in the spring) samples were examined. The samples were collected
totally from 139 animals because of the new animals were added due to death or slaughtering
in the spring period. 

            All
samples were enriched in modified Tryptic Soya Broth. After the immunomagnetic
separation, O157 Dynabeads and bacterial suspensions were inoculated onto
sorbitol MacConkey agar with cefixime and tellurite. Sorbitol negative colonies
were inoculated onto sorbitol MacConkey agar supplemented with
4-methylumbelliferyl-β-D-glucuronide. Suspected colonies which were chosen from
this medium were applied for latex and tube agglutination assays for
characterization of O157 serogroup, biochemical tests and tube agglutination
assay for detecting H7 antigen respectively.

            In
the fall period, E. coli O157 was
isolated from one environmental sample and E.coli
O157:H7 from one fecal sample. In the spring period, E. coli O157 was isolated from 10 and E. coli O157:H7 from one fecal sample. Seven of E. coli O157 isolates were from the same
farm which tested negative during in the first sampling. In total, 576 samples
were examined and two samples were identified as E. coli O157:H7 (0.35 %) and confirmed by PCR assay applied on the
BAX System Q7.

            E. coli O157:H7 which was identified in
the fall period was not isolated in the spring period. Because, the animal which
fecal sample taken from was newly added because of slaughtering or death.

            Seasonal
effect was detected significant only in the isolation rate of E. coli O157 in feces (p<0.01).

.

Article Details

Prevalence of E. coli O157:H7 in Dairy Cattle and the Farm Environment

Authors: Esin BİRDAL | Seyyal AK

Publisher: Bülent VEREP

Journal: Journal of Anatolian Environmental and Animal Sciences 2018 Vol.3 Issue 2, pp.85-90

Document Type: Article

Publication Date: 8/2018

Document Language: English

Document ID (Org No.): 2000491147

Frequency: Quarterly

eISSN: 2548-0006

DOI: https://doi.org/10.35229/jaes.387063

Issues

Authors:

Esin BİRDAL | Seyyal AK

Publisher:

Bülent VEREP

Journal:

Journal of Anatolian Environmental and Animal Sciences 2018 Vol.3 Issue 2, pp.85-90

Document Type:

Article

Publication Date:

8/2018

Document Language:

English

Document ID (Org No.):

2000491147

Frequency:

Quarterly

eISSN:

2548-0006

DOI:

https://doi.org/10.35229/jaes.387063