Outcomes of oxytocin treatment on intestinal ischemia-reperfusion injury in rats

Çağrı Gültekin; Serkan Sayıner; Şule Çetinel; Ahmet Özer Şehirli

Article Details

Outcomes of oxytocin treatment on intestinal ischemia-reperfusion injury in rats

Authors:

Çağrı Gültekin | Serkan Sayıner | Şule Çetinel | Ahmet Özer Şehirli

Publisher:

Ankara University

Main Topics:

Veterinary Surgery | Veterinary Biochemistry | Veterinary Pharmacology | Veterinary Histology and Embryology

Journal:

Ankara Üniversitesi Veteriner Fakültesi Dergisi Vol.71 Issue 3, pp.343-348

Document Type:

Article

Publication Date:

10/2024

Document Language:

English

Document ID (Org No.):

2001322311

pISSN:

1300-0861

Keywords:

Abstract:

 Ischemia-reperfusion injury is a clinical condition that poses life-threatening risks and can be caused by diseases or operations such as trauma, shock, and gastric dilatation volvulus. The objective of this study was to examine the effect of oxytocin on intestinal damage in rats induced by experimental ischemia-reperfusion injury. Three groups of Wistar albino rats were established: a control group CTR, n6, an intestinal ischemia-reperfusion group I-IR, n6, and an intestinal ischemia-reperfusion with oxytocin group I-IROxt, n6. The I-IROxt group received an intraperitoneal injection of 1 mgkg oxytocin 30 minutes before anesthesia. In the I-IR and I-IROxt groups, the superior mesenteric artery was ligated for 1 hour to induce ischemia-reperfusion injury, followed by one hour of reperfusion by opening the ligatures. At the end of the reperfusion period, the rats were euthanized, and blood and intestinal tissue samples were collected. From the blood samples, ALT, ALP, AST, LDH, BUN, creatinine, IL-1β, and TNF-α concentrations were evaluated. Tissue samples were analyzed for IL-1β, TNF-α, and MDA activity. Serum and tissue IL-1β and TNF-α concentrations were higher in both the I-IR and I-IROxt groups compared to the CTR group. However, these levels were found to be lower in the I-IROxt group compared to the I-IR group. The histopathological analysis showed that the I-IROxt group had better epithelial regeneration and less inflammatory cell infiltration compared to the I-IR group. In conclusion, oxytocin inhibited the release of IL-1β and TNF-α and the harmful effect of IR on intestinal cells.

 Ischemia-reperfusion injury is a clinical condition that poses life-threatening risks and can be caused by diseases or operations such as trauma, shock, and gastric dilatation volvulus. The objective of this study was to examine the effect of oxytocin on intestinal damage in rats induced by experimental ischemia-reperfusion injury. Three groups of Wistar albino rats were established: a control group CTR, n6, an intestinal ischemia-reperfusion group I-IR, n6, and an intestinal ischemia-reperfusion with oxytocin group I-IROxt, n6. The I-IROxt group received an intraperitoneal injection of 1 mgkg oxytocin 30 minutes before anesthesia. In the I-IR and I-IROxt groups, the superior mesenteric artery was ligated for 1 hour to induce ischemia-reperfusion injury, followed by one hour of reperfusion by opening the ligatures. At the end of the reperfusion period, the rats were euthanized, and blood and intestinal tissue samples were collected. From the blood samples, ALT, ALP, AST, LDH, BUN, creatinine, IL-1β, and TNF-α concentrations were evaluated. Tissue samples were analyzed for IL-1β, TNF-α, and MDA activity. Serum and tissue IL-1β and TNF-α concentrations were higher in both the I-IR and I-IROxt groups compared to the CTR group. However, these levels were found to be lower in the I-IROxt group compared to the I-IR group. The histopathological analysis showed that the I-IROxt group had better epithelial regeneration and less inflammatory cell infiltration compared to the I-IR group. In conclusion, oxytocin inhibited the release of IL-1β and TNF-α and the harmful effect of IR on intestinal cells.

 Ischemia-reperfusion injury is a clinical condition that poses life-threatening risks and can be caused by diseases or operations such as trauma, shock, and gastric dilatation volvulus. The objective of this study was to examine the effect of oxytocin on intestinal damage in rats induced by experimental ischemia-reperfusion injury. Three groups of Wistar albino rats were established: a control group CTR, n6, an intestinal ischemia-reperfusion group I-IR, n6, and an intestinal ischemia-reperfusion with oxytocin group I-IROxt, n6. The I-IROxt group received an intraperitoneal injection of 1 mgkg oxytocin 30 minutes before anesthesia. In the I-IR and I-IROxt groups, the superior mesenteric artery was ligated for 1 hour to induce ischemia-reperfusion injury, followed by one hour of reperfusion by opening the ligatures. At the end of the reperfusion period, the rats were euthanized, and blood and intestinal tissue samples were collected. From the blood samples, ALT, ALP, AST, LDH, BUN, creatinine, IL-1β, and TNF-α concentrations were evaluated. Tissue samples were analyzed for IL-1β, TNF-α, and MDA activity. Serum and tissue IL-1β and TNF-α concentrations were higher in both the I-IR and I-IROxt groups compared to the CTR group. However, these levels were found to be lower in the I-IROxt group compared to the I-IR group. The histopathological analysis showed that the I-IROxt group had better epithelial regeneration and less inflammatory cell infiltration compared to the I-IR group. In conclusion, oxytocin inhibited the release of IL-1β and TNF-α and the harmful effect of IR on intestinal cells.

 İskemi-reperfüzyon yaralanması, travma, şok, gastrik dilatasyon volvulus gibi bazı hastalıklar veya operasyonlar nedeniyle yaşamı tehdit eden bir klinik durumdur. Çalışmanın amacı, oksitosinin sıçanlarda deneysel olarak oluşturulan iskemi-reperfüzyon hasarının neden olduğu bağırsak hasarı üzerindeki etkisini araştırmaktı. Üç grup Wistar albino sıçan oluşturuldu: Kontrol CTR, n6, bağırsak iskemi-reperfüzyon I-IR, n6 ve bağırsak iskemi-reperfüzyon Oksitosin I-IROxt, n6. I-IROxt grubuna anesteziden 30 dakika önce intraperitoneal olarak oksitosin 1 mgkg verildi. I-IR ve I-IROxt gruplarında 1 saat superior mezenterik arter ligasyonu ve ligatürler açılarak 1 saat reperfüzyon ile iskemi reperfüzyonu uygulandı. Reperfüzyon süresinin bitiminden sonra ötenazi yapılarak kan ve barsak doku örnekleri alındı. Kan örneklerinden ALT, ALP, AST, LDH, BUN, kreatinin, IL-1β, TNF-α, konsantrasyonları ve doku örneğinden IL-1β, TNF-α ve MDA aktiviteleri değerlendirildi. Serum ve doku IL-1β, TNF-α konsantrasyonları hem I-IR hem de I-IROxt gruplarında CTR grubuna göre yüksek bulunurken, bu seviyelerin I-IROxt grubunda I-IROxt grubuna göre daha düşük olduğu belirlendi. I-IR grubu. Histopatolojik analiz, I-IR grubuna kıyasla I-IROxt grubunda hem epitel hem de bez yapısının epitelyal rejenerasyonunun ve inflamatuar hücre infiltrasyonunun azaldığını gösterdi. Sonuç olarak oksitosin, IL-1β ve TNF-α salınımını ve IRnin bağırsak hücreleri üzerindeki zararlı etkisini engellemiştir.

Article Details

Outcomes of oxytocin treatment on intestinal ischemia-reperfusion injury in rats

Authors:

Çağrı Gültekin | Serkan Sayıner | Şule Çetinel | Ahmet Özer Şehirli

Publisher:

Ankara University

Main Topics:

Veterinary Surgery | Veterinary Biochemistry | Veterinary Pharmacology | Veterinary Histology and Embryology

Journal:

Ankara Üniversitesi Veteriner Fakültesi Dergisi Vol.71 Issue 3, pp.343-348

Document Type:

Article

Publication Date:

10/2024

Document Language:

English

Document ID (Org No.):

2001322311

pISSN:

1300-0861

Cytokines

intestinal ischemia-reperfusion

oxytocin

ratİntestinal iskemi-reperfüzyon, oksitosin, sıçan, sitokinler

Issues

Article Details

Outcomes of oxytocin treatment on intestinal ischemia-reperfusion injury in rats

Authors: Çağrı Gültekin | Serkan Sayıner | Şule Çetinel | Ahmet Özer Şehirli

Publisher: Ankara University

Main Topics: Veterinary Surgery | Veterinary Biochemistry | Veterinary Pharmacology | Veterinary Histology and Embryology

Journal: Ankara Üniversitesi Veteriner Fakültesi Dergisi Vol.71 Issue 3, pp.343-348

Document Type: Article

Publication Date: 10/2024

Document Language: English

Document ID (Org No.): 2001322311

pISSN: 1300-0861

Cytokines

intestinal ischemia-reperfusion

oxytocin

ratİntestinal iskemi-reperfüzyon, oksitosin, sıçan, sitokinler

Issues

Authors:

Çağrı Gültekin | Serkan Sayıner | Şule Çetinel | Ahmet Özer Şehirli

Publisher:

Ankara University

Main Topics:

Veterinary Surgery | Veterinary Biochemistry | Veterinary Pharmacology | Veterinary Histology and Embryology

Journal:

Ankara Üniversitesi Veteriner Fakültesi Dergisi Vol.71 Issue 3, pp.343-348

Document Type:

Article

Publication Date:

10/2024

Document Language:

English

Document ID (Org No.):

2001322311

pISSN:

1300-0861