The Effects of Amitraz on the Motility of Guinea Pig Small Intestine

Vedat SAĞMANLIGİL; İlksin PİŞKİN; Meltem ŞİRELİ

Article Details

The Effects of Amitraz on the Motility of Guinea Pig Small Intestine

Authors:

Vedat SAĞMANLIGİL | İlksin PİŞKİN | Meltem ŞİRELİ

Publisher:

TUBITAK

Journal:

Turkish Journal of Veterinary and Animal Sciences 2001 Vol.25 Issue 1, pp.75-79

Document Type:

Article

Publication Date:

12/2000

Document Language:

Turkish

Document ID (Org No.):

2000771500

Frequency:

Bimonthly

eISSN:

1303-6181

pISSN:

1300-0128

Keywords:

Abstract:

 

In this study, 14 adult albino guinea pigs, weighing 300-350 g, were divided into two equal groups. The small intestinal  segments (distal ileum)taken from the animals were kept in vitro in the isolated organ bath, and the isotonic contractions of the  tissues produced by amitraz and acetylcholine were recorded on a polygraph. For the first group, tissues belonging to the seven  animals, the cumulative concentration-response curves of amitraz were obtained and compared by applications at concentrations  from 10-8 M to 10-3 M in three different conditions alone,in the presence of verapamil (3x10-5  M)and in Ca++  free solution. For  the second group, tissues taken from the other seven animals, the responses to acetylcholine (3x10-3  M)were compared with it given  alone (control), in the presence of amitraz at the effective dose for this tissue (10-3  M), verapamil (3x10-5  M)and finally in Ca++ free  solution.  The cumulative concentration-response curves of amitraz showed that amitraz at a concentration of 10-3  M created a contraction 3-  4-fold that of the others produced by a concentration of 10-8 -10-4 M. It was also observed that Ca++ played a big role in acetylcholine  and amitraz-induced contractions while the contraction produced by acetylcoline was not reduced by the effective concentration of  amitraz for the tissue.

 

In this study, 14 adult albino guinea pigs, weighing 300-350 g, were divided into two equal groups. The small intestinal  segments (distal ileum)taken from the animals were kept in vitro in the isolated organ bath, and the isotonic contractions of the  tissues produced by amitraz and acetylcholine were recorded on a polygraph. For the first group, tissues belonging to the seven  animals, the cumulative concentration-response curves of amitraz were obtained and compared by applications at concentrations  from 10-8 M to 10-3 M in three different conditions alone,in the presence of verapamil (3x10-5  M)and in Ca++  free solution. For  the second group, tissues taken from the other seven animals, the responses to acetylcholine (3x10-3  M)were compared with it given  alone (control), in the presence of amitraz at the effective dose for this tissue (10-3  M), verapamil (3x10-5  M)and finally in Ca++ free  solution.  The cumulative concentration-response curves of amitraz showed that amitraz at a concentration of 10-3  M created a contraction 3-  4-fold that of the others produced by a concentration of 10-8 -10-4 M. It was also observed that Ca++ played a big role in acetylcholine  and amitraz-induced contractions while the contraction produced by acetylcoline was not reduced by the effective concentration of  amitraz for the tissue.

Article Details

The Effects of Amitraz on the Motility of Guinea Pig Small Intestine

Authors:

Vedat SAĞMANLIGİL | İlksin PİŞKİN | Meltem ŞİRELİ

Publisher:

TUBITAK

Journal:

Turkish Journal of Veterinary and Animal Sciences 2001 Vol.25 Issue 1, pp.75-79

Document Type:

Article

Publication Date:

12/2000

Document Language:

Turkish

Document ID (Org No.):

2000771500

Frequency:

Bimonthly

eISSN:

1303-6181

pISSN:

1300-0128

Amitraz

small intestine

motility

guinea pig

Issues

Article Details

The Effects of Amitraz on the Motility of Guinea Pig Small Intestine

Authors: Vedat SAĞMANLIGİL | İlksin PİŞKİN | Meltem ŞİRELİ

Publisher: TUBITAK

Journal: Turkish Journal of Veterinary and Animal Sciences 2001 Vol.25 Issue 1, pp.75-79

Document Type: Article

Publication Date: 12/2000

Document Language: Turkish

Document ID (Org No.): 2000771500

Frequency: Bimonthly

eISSN: 1303-6181

pISSN: 1300-0128

Amitraz

small intestine

motility

guinea pig

Issues

Authors:

Vedat SAĞMANLIGİL | İlksin PİŞKİN | Meltem ŞİRELİ

Publisher:

TUBITAK

Journal:

Turkish Journal of Veterinary and Animal Sciences 2001 Vol.25 Issue 1, pp.75-79

Document Type:

Article

Publication Date:

12/2000

Document Language:

Turkish

Document ID (Org No.):

2000771500

Frequency:

Bimonthly

eISSN:

1303-6181

pISSN:

1300-0128