Piper sarmentosum attenuates dexamethasone-induced hypertension by stimulating endothelial nitric oxide synthase

Azizah Ugusman

Article Details

Piper sarmentosum attenuates dexamethasone-induced hypertension by stimulating endothelial nitric oxide synthase

Authors:

Azizah Ugusman

Publisher:

Marmara University

Main Topics:

Pharmacology and Pharmaceutical Sciences (Other)

Journal:

Journal of Research in Pharmacy Vol.24 Issue 1, pp.150-158

Document Type:

Article

Publication Date:

6/2025

Document Language:

English

Document ID (Org No.):

2001346760

pISSN:

2630-6344

Keywords:

Abstract:

 Reduced endothelial nitric oxide NO synthesized by endothelial nitric oxide synthase eNOS in the vasculature is a feature of endothelial dysfunction. Dexamethasone causes secondary hypertension by inhibiting eNOS activity. Piper sarmentosum is an herb with anti-hypertensive effect. The aim of this study was to evaluate the antihypertensive effect of aqueous extract of P. sarmentosum AEPS in dexamethasone Dex-induced hypertensive rats. A total of 30 male Sprague Dawley rats were divided into five groups including control, AEPS 500 mgkgday, orally, Dex 20 μgkgday, subcutaneously, Dex 20 μgkgday  AEPS 500 mgkgday and Dex 20 μgkgday  captopril 40 mgkgday, orally. Blood pressure was measured using tail-cuff method at baseline and fortnightly thereafter. The rats were sacrificed and the serum was collected to quantify the amount of NO after 28 days of treatment. Aortic samples were homogenized for measurement of eNOS mRNA expression, protein level and activity. Treatment of Dex-induced hypertensive rats with AEPS lowered systolic blood pressure P  0.001 and diastolic blood pressure P  0.01, increased eNOS mRNA expression P  0.01, eNOS protein P  0.01, eNOS activity P  0.05 and NO level P  0.05. In conclusion, AEPS reduces the blood pressure of Dex-induced hypertensive rats as it shows positive effects on eNOS and NO production.

 Reduced endothelial nitric oxide NO synthesized by endothelial nitric oxide synthase eNOS in the vasculature is a feature of endothelial dysfunction. Dexamethasone causes secondary hypertension by inhibiting eNOS activity. Piper sarmentosum is an herb with anti-hypertensive effect. The aim of this study was to evaluate the antihypertensive effect of aqueous extract of P. sarmentosum AEPS in dexamethasone Dex-induced hypertensive rats. A total of 30 male Sprague Dawley rats were divided into five groups including control, AEPS 500 mgkgday, orally, Dex 20 μgkgday, subcutaneously, Dex 20 μgkgday  AEPS 500 mgkgday and Dex 20 μgkgday  captopril 40 mgkgday, orally. Blood pressure was measured using tail-cuff method at baseline and fortnightly thereafter. The rats were sacrificed and the serum was collected to quantify the amount of NO after 28 days of treatment. Aortic samples were homogenized for measurement of eNOS mRNA expression, protein level and activity. Treatment of Dex-induced hypertensive rats with AEPS lowered systolic blood pressure P  0.001 and diastolic blood pressure P  0.01, increased eNOS mRNA expression P  0.01, eNOS protein P  0.01, eNOS activity P  0.05 and NO level P  0.05. In conclusion, AEPS reduces the blood pressure of Dex-induced hypertensive rats as it shows positive effects on eNOS and NO production.

 Reduced endothelial nitric oxide NO synthesized by endothelial nitric oxide synthase eNOS in the vasculature is a feature of endothelial dysfunction. Dexamethasone causes secondary hypertension by inhibiting eNOS activity. Piper sarmentosum is an herb with anti-hypertensive effect. The aim of this study was to evaluate the antihypertensive effect of aqueous extract of P. sarmentosum AEPS in dexamethasone Dex-induced hypertensive rats. A total of 30 male Sprague Dawley rats were divided into five groups including control, AEPS 500 mgkgday, orally, Dex 20 μgkgday, subcutaneously, Dex 20 μgkgday  AEPS 500 mgkgday and Dex 20 μgkgday  captopril 40 mgkgday, orally. Blood pressure was measured using tail-cuff method at baseline and fortnightly thereafter. The rats were sacrificed and the serum was collected to quantify the amount of NO after 28 days of treatment. Aortic samples were homogenized for measurement of eNOS mRNA expression, protein level and activity. Treatment of Dex-induced hypertensive rats with AEPS lowered systolic blood pressure P  0.001 and diastolic blood pressure P  0.01, increased eNOS mRNA expression P  0.01, eNOS protein P  0.01, eNOS activity P  0.05 and NO level P  0.05. In conclusion, AEPS reduces the blood pressure of Dex-induced hypertensive rats as it shows positive effects on eNOS and NO production.

 Reduced endothelial nitric oxide NO synthesized by endothelial nitric oxide synthase eNOS in the vasculature is a feature of endothelial dysfunction. Dexamethasone causes secondary hypertension by inhibiting eNOS activity. Piper sarmentosum is an herb with anti-hypertensive effect. The aim of this study was to evaluate the antihypertensive effect of aqueous extract of P. sarmentosum AEPS in dexamethasone Dex-induced hypertensive rats. A total of 30 male Sprague Dawley rats were divided into five groups including control, AEPS 500 mgkgday, orally, Dex 20 μgkgday, subcutaneously, Dex 20 μgkgday  AEPS 500 mgkgday and Dex 20 μgkgday  captopril 40 mgkgday, orally. Blood pressure was measured using tail-cuff method at baseline and fortnightly thereafter. The rats were sacrificed and the serum was collected to quantify the amount of NO after 28 days of treatment. Aortic samples were homogenized for measurement of eNOS mRNA expression, protein level and activity. Treatment of Dex-induced hypertensive rats with AEPS lowered systolic blood pressure P  0.001 and diastolic blood pressure P  0.01, increased eNOS mRNA expression P  0.01, eNOS protein P  0.01, eNOS activity P  0.05 and NO level P  0.05. In conclusion, AEPS reduces the blood pressure of Dex-induced hypertensive rats as it shows positive effects on eNOS and NO production.

Article Details

Piper sarmentosum attenuates dexamethasone-induced hypertension by stimulating endothelial nitric oxide synthase

Authors: Azizah Ugusman

Publisher: Marmara University

Main Topics: Pharmacology and Pharmaceutical Sciences (Other)

Journal: Journal of Research in Pharmacy Vol.24 Issue 1, pp.150-158

Document Type: Article

Publication Date: 6/2025

Document Language: English

Document ID (Org No.): 2001346760

pISSN: 2630-6344

Dexamethasone

endothelial nitric oxide synthase

hypertension

nitric oxide

Piper sarmentosum

Issues

Authors:

Azizah Ugusman

Publisher:

Marmara University

Main Topics:

Pharmacology and Pharmaceutical Sciences (Other)

Journal:

Journal of Research in Pharmacy Vol.24 Issue 1, pp.150-158

Document Type:

Article

Publication Date:

6/2025

Document Language:

English

Document ID (Org No.):

2001346760

pISSN:

2630-6344