Targeting the tumor metabolism by oxamate potentiates the impact of chemotherapeutics in colorectal cancer cells

Gizem ÇALIBAŞI KOÇAL; Çağrı ÇAKICI; Feriha TOKSÖZ; Şeniz İNANÇ SÜRER; Tolga SEVER; Yasemin BAŞBINAR; Türkan YİĞİTBAŞI

doi:https://doi.org/10.30621/jbachs.988996

Article Details

Targeting the tumor metabolism by oxamate potentiates the impact of chemotherapeutics in colorectal cancer cells

Authors:

Gizem ÇALIBAŞI KOÇAL | Çağrı ÇAKICI | Feriha TOKSÖZ | Şeniz İNANÇ SÜRER | Tolga SEVER | Yasemin BAŞBINAR | Türkan YİĞİTBAŞI

Publisher:

Dokuz Eylul University

Main Topics:

Promotion of health

Journal:

Journal of Basic and Clinical Health Sciences 2021 Vol.5 Issue 3, pp.205-212

Document Type:

Article

Publication Date:

9/2021

Document Language:

English

Document ID (Org No.):

2000493163

Frequency:

Tri-annual

eISSN:

2564-7288

DOI:

https://doi.org/10.30621/jbachs.988996

Abstract:

 

Background/Aim: Cancer cells promote lactate formation via pyruvate rather than oxidative phosphorylation by programming their metabolism to maintain proliferation under the Warburg effect. It has shown that the altered metabolic phenotype with activation of lactate dehydrogenase-A in the cancer cell may affect survival, chemotherapy resistance, and metastasis. In this direction, studies are focusing on reprogramming cancer metabolism and increase the effectiveness of chemotherapy. In this study, the main aim was to target the Warburg phenotype via the inhibition of lactate dehydrogenase with the combination of sodium oxamate and current colorectal cancer treatment options such as 5-fluorouracil and irinotecan. 
Methods: The effect of chemotherapeutics on the cellular behaviour was evaluated by real-time cytotoxicity and migration analysis systems, and metabolic phenotype was assessed by measuring lactate, lactate dehydrogenase expression, and reactive oxygen species levels. 
Results: According to the results, the viability and migration of colorectal cancer cells were significantly decreased with the combination of chemotherapeutics and sodium oxamate which decreases lactate levels. 
Conclusion: As a result, the combination of sodium oxamate with the chemotherapeutics hinders the cancer cell viability and migration by changing metabolic phenotype with decreased lactate.

Article Details

Targeting the tumor metabolism by oxamate potentiates the impact of chemotherapeutics in colorectal cancer cells

Authors: Gizem ÇALIBAŞI KOÇAL | Çağrı ÇAKICI | Feriha TOKSÖZ | Şeniz İNANÇ SÜRER | Tolga SEVER | Yasemin BAŞBINAR | Türkan YİĞİTBAŞI

Publisher: Dokuz Eylul University

Main Topics: Promotion of health

Journal: Journal of Basic and Clinical Health Sciences 2021 Vol.5 Issue 3, pp.205-212

Document Type: Article

Publication Date: 9/2021

Document Language: English

Document ID (Org No.): 2000493163

Frequency: Tri-annual

eISSN: 2564-7288

DOI: https://doi.org/10.30621/jbachs.988996

Issues

Authors:

Gizem ÇALIBAŞI KOÇAL | Çağrı ÇAKICI | Feriha TOKSÖZ | Şeniz İNANÇ SÜRER | Tolga SEVER | Yasemin BAŞBINAR | Türkan YİĞİTBAŞI

Publisher:

Dokuz Eylul University

Main Topics:

Promotion of health

Journal:

Journal of Basic and Clinical Health Sciences 2021 Vol.5 Issue 3, pp.205-212

Document Type:

Article

Publication Date:

9/2021

Document Language:

English

Document ID (Org No.):

2000493163

Frequency:

Tri-annual

eISSN:

2564-7288

DOI:

https://doi.org/10.30621/jbachs.988996