Quantitative Analysis of Ciprofloxacin in an Ophthalmic Solution Using UV Absorption Spectrophotometry and Derivative Spectrophotometry

Saadet DERMİŞ; Sümeyye KILIÇ; Zehra Ceren ERTEKİN; Erdal DİNÇ

doi:https://doi.org/10.29233/sdufeffd.458082

Article Details

Quantitative Analysis of Ciprofloxacin in an Ophthalmic Solution Using UV Absorption Spectrophotometry and Derivative Spectrophotometry

Authors:

Saadet DERMİŞ | Sümeyye KILIÇ | Zehra Ceren ERTEKİN | Erdal DİNÇ

Publisher:

Süleyman Demirel University

Main Topics:

Chemical engineering

Journal:

Süleyman Demirel University Faculty of Arts and Science Journal of Science 2019 Vol.14 Issue 1, pp.71-76

Document Type:

Article

Publication Date:

5/2019

Document Language:

English

Document ID (Org No.):

2000582601

Frequency:

Bi-Annual

eISSN:

1306-7575

DOI:

https://doi.org/10.29233/sdufeffd.458082

Abstract:

 


The
quantitative analysis of ciprofloxacin in an ophthalmic solution was performed
by UV absorption spectrophotometry and first derivative spectrophotometry. The
UV absorption spectra of standard series and samples containing ciprofloxacin
were recorded. Then, the derivative of UV absorption spectra was obtained for
the derivative spectrophotometric analysis of the related drug.  Linear regression equations in the linear concentration
range of 3.0-28.0 µg/mL for analysis of ciprofloxacin were obtained by using
direct absorbance measurement at 278.9 nm and using the dA/dλ values at 283.2
nm. The recovery assay results were found between 97.8 to 102.0 %. It was
observed that the limits of detection and quantification were 0.74 and 2.74
μg/mL for UV absorption spectrophotometry and 0.60 and 2.00 μg/mL for first
derivative spectrophotometry, respectively. Analysis results indicated that the
proposed methods were precise, accurate and reliable for the determination of
ciprofloxacin in commercial samples.

.

 


Bu
çalışmada oftalmik çözeltide siprofloksasinin kantitatif tayini için UV
absorbans spektrofotometrisi ve türev spektrofotometrisi kullanılmıştır.
Siprofloksasin içeren standart serinin ve numunelerin UV absorbans spektrumları
kaydedilmiştir. Daha sonra türev spektrofotometrisi için, UV absorbans spektrumunun
birinci türevi alınmıştır. 3.0-28.0 µg/mL doğrusal çalışma aralığında olmak
üzere, 278.9 nm’deki absorbans değerleri, ve 283.2 nm’deki dA/dλ değerleri
kullanılarak lineer regresyon eşitlikleri elde edilmiştir. Geri kazanım
çalışmaları sonuçları % 97.8 ile % 102.0
arasında hesaplanmıştır. Yakalama ve tayin sınır değerleri, UV absorbans
spektrofotometrisi için 0.74 µg/mL ve 2.74
µg/mL; türev spektrofotometrisi için 0.60 µg/mL
ve 2.00 µg/mL olarak hesaplanmıştır. Sonuçlar, siprofloksasinin ticari örneklerden
analizi için önerilen yöntemlerin kesin, doğru ve güvenilir olduğunu
göstermiştir. 

.

Article Details

Quantitative Analysis of Ciprofloxacin in an Ophthalmic Solution Using UV Absorption Spectrophotometry and Derivative Spectrophotometry

Authors: Saadet DERMİŞ | Sümeyye KILIÇ | Zehra Ceren ERTEKİN | Erdal DİNÇ

Publisher: Süleyman Demirel University

Main Topics: Chemical engineering

Journal: Süleyman Demirel University Faculty of Arts and Science Journal of Science 2019 Vol.14 Issue 1, pp.71-76

Document Type: Article

Publication Date: 5/2019

Document Language: English

Document ID (Org No.): 2000582601

Frequency: Bi-Annual

eISSN: 1306-7575

DOI: https://doi.org/10.29233/sdufeffd.458082

Issues

Authors:

Saadet DERMİŞ | Sümeyye KILIÇ | Zehra Ceren ERTEKİN | Erdal DİNÇ

Publisher:

Süleyman Demirel University

Main Topics:

Chemical engineering

Journal:

Süleyman Demirel University Faculty of Arts and Science Journal of Science 2019 Vol.14 Issue 1, pp.71-76

Document Type:

Article

Publication Date:

5/2019

Document Language:

English

Document ID (Org No.):

2000582601

Frequency:

Bi-Annual

eISSN:

1306-7575

DOI:

https://doi.org/10.29233/sdufeffd.458082