In Vitro Differentiation of Embryonic Stem Cells to Muscle Like Cells after Treatment with Muscle Extract

Intissar N. Waheed

Article Details

In Vitro Differentiation of Embryonic Stem Cells to Muscle Like Cells after Treatment with Muscle Extract

Authors:

Intissar N. Waheed

Publisher:

The Hashemite University : The Deanship of Scientific Research and Graduate Studies

Main Topics:

Life sciences

Journal:

Jordan Journal of Biological Sciences 2011 Vol.4 Issue 3, pp.177-184

Document Type:

Article

Publication Date:

9/2011

Document Language:

English

Document ID (Org No.):

27928

Frequency:

Quarterly

pISSN:

19956673

Descriptors:

Keywords:

Abstract:

 One goal of embryonic stem cells (ESCs) research is the development of specialized cells such as neurons, heart musclecells, endothelial cells of blood vessels, etc. Thus, the directed derivation of ESCs is vital to the ultimate use of such cells inthe development of new therapies. This study was conducted to describe the conditions that induce differentiation of innercell mass (ICM) derived ESCs into muscle like cells. Blastocysts were recovered from female's albino mice of strain Balb \cat day 3.5 after natural mating had taken place, cultured in MEM medium supplemented with 20% FCS in the presence ofmitotically inactivated feeder layer attached to gelatin layer as a substrate at 37C˚ and 5% CO2. Within two days of theculture, the blastocyst were erupted from the Zona Pellucid and attached by their equatorial pole to the underlying substrateand the ICM, visible as a round dense cell mass in the center of the outgrowth of the trophoblastic cells. Within seven days,the ICM proliferated and increased in their girth, and numbers of ESCs were observed to be librated from the ICM to thesurrounding area. When these ESCs were treated with mouse embryonic skeletal muscle extract (ESME) for twelve days,they differentiated and appeared as smooth muscle like cells and expressed positive response against primary monoclonalantibody anti myosin. We can conclude that mouse ESME plays an important role to direct differentiation of ESCs to themuscular pathway.

Article Details

In Vitro Differentiation of Embryonic Stem Cells to Muscle Like Cells after Treatment with Muscle Extract

Authors:

Intissar N. Waheed

Publisher:

The Hashemite University : The Deanship of Scientific Research and Graduate Studies

Main Topics:

Life sciences

Journal:

Jordan Journal of Biological Sciences 2011 Vol.4 Issue 3, pp.177-184

Document Type:

Article

Publication Date:

9/2011

Document Language:

English

Document ID (Org No.):

27928

Frequency:

Quarterly

pISSN:

19956673

Descriptors:

STEM CELLS

CELL DIFFERENTIATION

EMBRYONIC DEVELOPMENT

MUSCLES

GENES

Inner cell mass

embryonic stem cell

myogenic differentiation

anti-myosin

Issues

Article Details

Authors: Intissar N. Waheed

Publisher: The Hashemite University : The Deanship of Scientific Research and Graduate Studies

Main Topics: Life sciences

Journal: Jordan Journal of Biological Sciences 2011 Vol.4 Issue 3, pp.177-184

Document Type: Article

Publication Date: 9/2011

Document Language: English

Document ID (Org No.): 27928

Frequency: Quarterly

pISSN: 19956673

Descriptors:

Issues

Authors:

Intissar N. Waheed

Publisher:

The Hashemite University : The Deanship of Scientific Research and Graduate Studies

Main Topics:

Life sciences

Journal:

Jordan Journal of Biological Sciences 2011 Vol.4 Issue 3, pp.177-184

Document Type:

Article

Publication Date:

9/2011

Document Language:

English

Document ID (Org No.):

27928

Frequency:

Quarterly

pISSN:

19956673