Multiple Shoot Regeneration from Shoot Tip and Nodal Explants of Rotala rotundifolia (Buch-Ham. ex Roxb) Koehne

Muhammet DOGAN

doi:https://doi.org/10.30616/ajb.343304

Article Details

Multiple Shoot Regeneration from Shoot Tip and Nodal Explants of Rotala rotundifolia (Buch-Ham. ex Roxb) Koehne

Authors:

Muhammet DOGAN

Publisher:

Abdullah KAYA

Main Topics:

Life sciences

Journal:

Anatolian Journal of Botany 2017 Vol.1 Issue 1, pp.4-8

Document Type:

Article

Publication Date:

10/2017

Document Language:

Turkish

Document ID (Org No.):

2000882277

Frequency:

Bi-Annual

eISSN:

2602-2818

DOI:

https://doi.org/10.30616/ajb.343304

Abstract:

 

Rotala
rotundifolia (Buch-Ham. ex Roxb) Koehne, an
aquatic plant belonging to the family Lythraceae, is used for the treatment of some
diseases due to its medical and anti-microbial properties. This study presents
multiple shoot regeneration from shoot tip and nodal explants of R.
rotundifolia cultured on Murashige and Skoog (MS) nutrient medium
containing 0.05-1.25 mg/L Kinetin (KIN) and 0.25 mg/L Gibberellic acid (GA3)
combinations for eight weeks. At the end of the second week, shoot formations
began to be observed on the explants. High shoot regeneration frequencies were
determined for both explants in the culture medium. The maximum number
of shoots per explant was obtained from shoot tip (38.66)
and nodal (30.77) explants cultured on MS medium containing 0.25 mg/L KIN +
0.25 mg/L GA3. Whereas the minimum number of shoots per explant was determined
on MS medium containing 1.25 mg/L KIN + 0.25 mg/L GA3 for both
explant types.The highest shoot lengths for shoot tip (1.87 cm) and
nodal (1.79 cm) explants were obtained on MS culture medium containing 0.75
mg/L KIN + 0.25 mg/L GA3 and 0.50 mg/L KIN + 0.25 mg/L GA3,
respectively. For in vitro rooting of
the regenerated shoots, 2 cm long cut shoots were transferred to MS medium
containing 0.25 mg/L indole-3-butyric acid (IBA). The rooted shoots were then
successfully acclimatized to external conditions in the aquarium environment. .

Article Details

Multiple Shoot Regeneration from Shoot Tip and Nodal Explants of Rotala rotundifolia (Buch-Ham. ex Roxb) Koehne

Authors: Muhammet DOGAN

Publisher: Abdullah KAYA

Main Topics: Life sciences

Journal: Anatolian Journal of Botany 2017 Vol.1 Issue 1, pp.4-8

Document Type: Article

Publication Date: 10/2017

Document Language: Turkish

Document ID (Org No.): 2000882277

Frequency: Bi-Annual

eISSN: 2602-2818

DOI: https://doi.org/10.30616/ajb.343304

Issues

Authors:

Muhammet DOGAN

Publisher:

Abdullah KAYA

Main Topics:

Life sciences

Journal:

Anatolian Journal of Botany 2017 Vol.1 Issue 1, pp.4-8

Document Type:

Article

Publication Date:

10/2017

Document Language:

Turkish

Document ID (Org No.):

2000882277

Frequency:

Bi-Annual

eISSN:

2602-2818

DOI:

https://doi.org/10.30616/ajb.343304