Expression of vascular endothelial growth factor and hypoxia-inducible factor-1 alpha during the periovulatory period in goats

Chainarong NAVANUKRAW; Jiratti THAMMASIRI; Tossapol MOONMANEE; Jaruk NATTHAKORNKUL

Article Details

Expression of vascular endothelial growth factor and hypoxia-inducible factor-1 alpha during the periovulatory period in goats

Authors:

Chainarong NAVANUKRAW | Jiratti THAMMASIRI | Tossapol MOONMANEE | Jaruk NATTHAKORNKUL

Publisher:

TUBITAK

Journal:

Turkish Journal of Veterinary and Animal Sciences 2014 Vol.38 Issue 6, pp.699-706

Document Type:

Article

Publication Date:

5/2014

Document Language:

Turkish

Document ID (Org No.):

2000765818

Frequency:

Bimonthly

eISSN:

1303-6181

pISSN:

1300-0128

Keywords:

Abstract:

 

To evaluate the expression of vascular endothelial growth factor (VEGF) and hypoxia-inducible factor-1 alpha (HIF-1 alpha) during the periovulatory period, goat ovaries were collected at 0, 4, 8, 12, 24, and 48 h after treatment with 300 IU hCG. Total cellular RNA was isolated and VEGF and HIF-1 alpha were quantified using real-time RT-PCR. The mRNA levels of VEGF in thecal tissue remained unchanged prior to ovulation. However, the mRNA levels of VEGF in thecal tissues at 48 h increased to 7.5-fold of the level at 0 h (P < 0.05). In granulosa tissue, the VEGF mRNA levels were not detected in granulosa cells at 48 h. In thecal tissue, the mRNA level of HIF-1 1 alpha at 4 h was 1.5-fold of the level at 0 h and remained unchanged during 8-24 h; however the levels were significantly increased at 48 h. The mRNA levels of HIF-1 alpha in granulosa at 4 h was not changed but levels were increased (P < 0.05) at 8 h and at 12 h and then decreased at 24 and 48 h, respectively. These data describe the relationships of VEGF- and HIF-1 alpha -dependent angiogenesis during the periovulatory periods by which hypoxia during ovulation is crucial for establishing the thecal new vasculature.

 

To evaluate the expression of vascular endothelial growth factor (VEGF) and hypoxia-inducible factor-1 alpha (HIF-1 alpha) during the periovulatory period, goat ovaries were collected at 0, 4, 8, 12, 24, and 48 h after treatment with 300 IU hCG. Total cellular RNA was isolated and VEGF and HIF-1 alpha were quantified using real-time RT-PCR. The mRNA levels of VEGF in thecal tissue remained unchanged prior to ovulation. However, the mRNA levels of VEGF in thecal tissues at 48 h increased to 7.5-fold of the level at 0 h (P < 0.05). In granulosa tissue, the VEGF mRNA levels were not detected in granulosa cells at 48 h. In thecal tissue, the mRNA level of HIF-1 1 alpha at 4 h was 1.5-fold of the level at 0 h and remained unchanged during 8-24 h; however the levels were significantly increased at 48 h. The mRNA levels of HIF-1 alpha in granulosa at 4 h was not changed but levels were increased (P < 0.05) at 8 h and at 12 h and then decreased at 24 and 48 h, respectively. These data describe the relationships of VEGF- and HIF-1 alpha -dependent angiogenesis during the periovulatory periods by which hypoxia during ovulation is crucial for establishing the thecal new vasculature.

Article Details

Expression of vascular endothelial growth factor and hypoxia-inducible factor-1 alpha during the periovulatory period in goats

Authors:

Chainarong NAVANUKRAW | Jiratti THAMMASIRI | Tossapol MOONMANEE | Jaruk NATTHAKORNKUL

Publisher:

TUBITAK

Journal:

Turkish Journal of Veterinary and Animal Sciences 2014 Vol.38 Issue 6, pp.699-706

Document Type:

Article

Publication Date:

5/2014

Document Language:

Turkish

Document ID (Org No.):

2000765818

Frequency:

Bimonthly

eISSN:

1303-6181

pISSN:

1300-0128

Vascular endothelial growth factor

hypoxia-inducible factor-1 alpha

periovulatory period

goats

Issues

Article Details

Expression of vascular endothelial growth factor and hypoxia-inducible factor-1 alpha during the periovulatory period in goats

Authors: Chainarong NAVANUKRAW | Jiratti THAMMASIRI | Tossapol MOONMANEE | Jaruk NATTHAKORNKUL

Publisher: TUBITAK

Journal: Turkish Journal of Veterinary and Animal Sciences 2014 Vol.38 Issue 6, pp.699-706

Document Type: Article

Publication Date: 5/2014

Document Language: Turkish

Document ID (Org No.): 2000765818

Frequency: Bimonthly

eISSN: 1303-6181

pISSN: 1300-0128

Vascular endothelial growth factor

hypoxia-inducible factor-1 alpha

periovulatory period

goats

Issues

Authors:

Chainarong NAVANUKRAW | Jiratti THAMMASIRI | Tossapol MOONMANEE | Jaruk NATTHAKORNKUL

Publisher:

TUBITAK

Journal:

Turkish Journal of Veterinary and Animal Sciences 2014 Vol.38 Issue 6, pp.699-706

Document Type:

Article

Publication Date:

5/2014

Document Language:

Turkish

Document ID (Org No.):

2000765818

Frequency:

Bimonthly

eISSN:

1303-6181

pISSN:

1300-0128