Effects of Short-term High Glucose on NIH/3T3 Fibroblast Proliferation, Apoptosis, and Collagen Type I Production

Tuğba SOYDAŞ; Elif YAPRAK SARAÇ; Suzan ÇINAR; Güven YENMİŞ; Sibel DOĞAN; Seyhun SOLAKOĞLU; Matem TUNÇDEMİR; Gönül KANIGÜR SULTUYBEK

Article Details

Effects of Short-term High Glucose on NIH/3T3 Fibroblast Proliferation, Apoptosis, and Collagen Type I Production

Authors:

Tuğba SOYDAŞ | Elif YAPRAK SARAÇ | Suzan ÇINAR | Güven YENMİŞ | Sibel DOĞAN | Seyhun SOLAKOĞLU | Matem TUNÇDEMİR | Gönül KANIGÜR SULTUYBEK

Publisher:

Istanbul Aydin University

Main Topics:

Diseases

Journal:

Journal of Medical Clinics 2019 Vol.2 Issue 3, pp.91-95

Document Type:

Article

Publication Date:

8/2019

Document Language:

English

Document ID (Org No.):

2000803266

Frequency:

Monthly

eISSN:

2687-217X

pISSN:

2630-5585

Keywords:

Abstract:

 


Objective: To investigate the mechanisms of in vitro high glucose induced model of liverfibrosis.Material and methods: The effects of high glucose concentration on fibroblast proliferationwere investigated by BrdU immunostaining. Apoptosis and necrosis levels were analyzed byflow cytometric assay. The content of collagen type I was measured by Collagen EstimationAssay through ELISA.Results: A high glucose medium not only increased NIH/3T3 fibroblast proliferation, but alsoincreased type I collagen synthesis, showing a similar condition to the fibrosis. Moreover, thehigh glucose caused an increased level of cellular apoptosis and necrosis.Conclusions: High glucose modulates the fibrosis in NIH/3T3 fibroblast cells via inducing theproduction of type I collagen while maintains the homeostasis by inducing the apoptosis andnecrosis of cells.

.

 


Amaç: Yüksek glikoza bağlı karaciğer fibrozis mekanizmalarını in vitro model kullanarakaraştırmak amaçlanmıştır.Gereç ve yöntem: Yüksek glikoz konsantrasyonunun fibroblast proliferasyonu üzerindekietkileri BrdU immünositokimya tekniği ile incelenmiştir. Apoptoz seviyeleri ve nekroz akımsitometri analizi ile tespit edildi. Kollajen tip I içeriği, ELISA ile ölçüldü.Bulgular: Yüksek glikoz düzeyi sadece NIH/3T3 fibroblast proliferasyonunu arttırmadı, aynızamanda fibrozise benzer bir durumun göstergesi olarak tip I kollajen sentezini de arttırdı.Ayrıca yüksek glikoz, apoptoz ve nekroz seviyelerinin artmasına da neden olmuştur.Sonuç: Yüksek glikoz, NIH/3T3 fibroblast hücrelerinde fibrozisi, tip I kollajen üretiminiindükleyerek modüle ederken, hücrelerin apoptoz ve nekrozunu indükleyerek homeostazı korur.

.

Article Details

Effects of Short-term High Glucose on NIH/3T3 Fibroblast Proliferation, Apoptosis, and Collagen Type I Production

Authors: Tuğba SOYDAŞ | Elif YAPRAK SARAÇ | Suzan ÇINAR | Güven YENMİŞ | Sibel DOĞAN | Seyhun SOLAKOĞLU | Matem TUNÇDEMİR | Gönül KANIGÜR SULTUYBEK

Publisher: Istanbul Aydin University

Main Topics: Diseases

Journal: Journal of Medical Clinics 2019 Vol.2 Issue 3, pp.91-95

Document Type: Article

Publication Date: 8/2019

Document Language: English

Document ID (Org No.): 2000803266

Frequency: Monthly

eISSN: 2687-217X

pISSN: 2630-5585

High glucose

Collagen

NIH/3T3 fibroblast

Apoptosis

Issues

Authors:

Tuğba SOYDAŞ | Elif YAPRAK SARAÇ | Suzan ÇINAR | Güven YENMİŞ | Sibel DOĞAN | Seyhun SOLAKOĞLU | Matem TUNÇDEMİR | Gönül KANIGÜR SULTUYBEK

Publisher:

Istanbul Aydin University

Main Topics:

Diseases

Journal:

Journal of Medical Clinics 2019 Vol.2 Issue 3, pp.91-95

Document Type:

Article

Publication Date:

8/2019

Document Language:

English

Document ID (Org No.):

2000803266

Frequency:

Monthly

eISSN:

2687-217X

pISSN:

2630-5585