CTLA-4 Gene Polymorphism in Relation to Thyroid Disorders in Patients with Chronic Hepatitis C Under Interferon Alpha Therapy

Salwa M. Abo El-Khair; Abd El-Hameed A. Metwali; Mohamed Abd El-Maksoud; Tarek Besheer

doi:10.3844/ajbbsp.2012.179.194

Article Details

CTLA-4 Gene Polymorphism in Relation to Thyroid Disorders in Patients with Chronic Hepatitis C Under Interferon Alpha Therapy

Authors:

Salwa M. Abo El-Khair | Abd El-Hameed A. Metwali | Mohamed Abd El-Maksoud | Tarek Besheer

Publisher:

Science Publications

Journal:

American Journal of Biochemistry and Biotechnology Vol.8 Issue 3, pp.179-194

Document Type:

Article

Publication Date:

9/2012

Document Language:

English

Document ID (Org No.):

2001397110

pISSN:

1558-6332

DOI:

10.3844/ajbbsp.2012.179.194

Keywords:

Abstract:

 Thyroid autoimmunity and dysfunction have reported as side effects of interferon-α treatment. The CT60 and exon 1+49 A/G polymorphisms in the Cytotoxic T-Lymphocyte Antigen 4 (CTLA-4) gene have linked to susceptibility to autoimmune disease. The aim of this research was to analyze the frequencies of CTLA-4 CT60 and +49 A/G polymorphisms and evaluate both polymorphisms as indicators of thyroid disorder susceptibility in chronic HCV Egyptian patients under interferon therapy. This study was carried out on 114 chronic HCV patients under combined therapy of interferon-α and Ribavirin. From them, 60 chronic HCV patients without thyroid disorder were considered the control group. The other 54 patients were having thyroid disorder either hypothyroidism (N = 35) or Grave’s Disease (GD) (N = 19). For all subjects, the genotypes of CTLA-4 gene CT60 and +49 A/G polymorphisms were studied using RFLP technique. For +49 A/G polymorphism, the genotypes frequencies in controls were: AA (30), AG (31.7) and GG (38.3%). Whereas, in hypothyroidism patients, the AA, AG and GG genotypes frequencies were (20), (57.1%) and (22.9%) respectively, while in grave’s disease, the AA, AG and GG genotypes frequencies were (26.3 (57.9) and (15.8%) respectively. The AG genotype was significantly associated with thyroid disease. As regards CT60 polymorphism, the genotypes frequencies in controls were: CC (38.3), CT (35) and TT (26.7%). Whereas, in hypothyroidism patients, the CC, CT and TT genotypes frequencies were (60), (20) and (20%) respectively, while in grave’s disease, the CC, CT and TT genotypes frequencies were (68.4), (15.8) and (15.8%) respectively. The CC genotype was significantly associated with thyroid disorders. CTLA-4 gene +49A/G and CT60 polymorphisms confer susceptibility to autoimmune thyroid disorder and confirm usefulness of CTLA-4 genotyping in predicting thyroid disorder in chronic HCV patients under interferon therapy.

Article Details

CTLA-4 Gene Polymorphism in Relation to Thyroid Disorders in Patients with Chronic Hepatitis C Under Interferon Alpha Therapy

Authors:

Salwa M. Abo El-Khair | Abd El-Hameed A. Metwali | Mohamed Abd El-Maksoud | Tarek Besheer

Publisher:

Science Publications

Journal:

American Journal of Biochemistry and Biotechnology Vol.8 Issue 3, pp.179-194

Document Type:

Article

Publication Date:

9/2012

Document Language:

English

Document ID (Org No.):

2001397110

pISSN:

1558-6332

DOI:

10.3844/ajbbsp.2012.179.194

Cytotoxic T-Lymphocyte Antigen 4

+49A/G

CT60

RFLP

Autoimmune Disease

Hepatitis C Virus

Issues

Article Details

CTLA-4 Gene Polymorphism in Relation to Thyroid Disorders in Patients with Chronic Hepatitis C Under Interferon Alpha Therapy

Authors: Salwa M. Abo El-Khair | Abd El-Hameed A. Metwali | Mohamed Abd El-Maksoud | Tarek Besheer

Publisher: Science Publications

Journal: American Journal of Biochemistry and Biotechnology Vol.8 Issue 3, pp.179-194

Document Type: Article

Publication Date: 9/2012

Document Language: English

Document ID (Org No.): 2001397110

pISSN: 1558-6332

DOI: 10.3844/ajbbsp.2012.179.194

Cytotoxic T-Lymphocyte Antigen 4

+49A/G

CT60

RFLP

Autoimmune Disease

Hepatitis C Virus

Issues

Authors:

Salwa M. Abo El-Khair | Abd El-Hameed A. Metwali | Mohamed Abd El-Maksoud | Tarek Besheer

Publisher:

Science Publications

Journal:

American Journal of Biochemistry and Biotechnology Vol.8 Issue 3, pp.179-194

Document Type:

Article

Publication Date:

9/2012

Document Language:

English

Document ID (Org No.):

2001397110

pISSN:

1558-6332

DOI:

10.3844/ajbbsp.2012.179.194