Molecular phylogenetic analysis of the taxa belonging to the genus Carlina L.(Asteraceae) in Turkey

Berna Sanön

Article Details

Molecular phylogenetic analysis of the taxa belonging to the genus Carlina L.(Asteraceae) in Turkey

Authors:

Berna Sanön

Publisher:

Balıkesir University

Main Topics:

Bitki Morfolojisi ve Anatomisi

Journal:

Balıkesir Üniversitesi Fen Bilimleri Enstitüsü Dergisi Vol.26 Issue 1, pp.249-259

Document Type:

Article

Publication Date:

1/2024

Document Language:

English

Document ID (Org No.):

2001303636

pISSN:

1301-7985

Keywords:

Abstract:

 Asteraceae, is a cosmopolitan family in terms of natural chemistry, flowering morphology, and adaptation to habitat. Even if there are numerous taxonomic and new molecular phylogenetic studies, the ancestors of the Asteraceae family are not well defined. Study material under consideration, Carlina L., is a member of the tribe Cardueae which belong to Cichorioideaea subfamily of the Asteraceae. The genus Carlina is represented by C. lanata, C. vulgaris, C. tragacanthifolia, C. biebersteinii, C. intermedia, C. oligocephala, C. involucrata subsp. libanotica, C. corymbosa, C.graeca in The Flora of Turkey. In this study, molecular phylogenetic analysis of the genus Carlina, which has a natural distribution in Turkey, has been made. The DNA isolation was performed using phenol- chloroform- isoamylalcohol. ITS Internal Transcribed Region of the nuclear ribosomal DNA nrDNA and the trnL-F Transfer RNA coding region of the chloroplast DNA sequences were used as molecular markers. Carlina was selected as ingroup taxon and Cardopatium, Atractylis and Carthamus were outgroup taxa. Taxa belonging to the genus Carlina distributed in Turkey were analyzed phylogenetically using Branch-and-Bound algorithm with maximum parsimony criterion. Data set analysis using 952 best parsimony trees was made. One tree topology was the most reliable and clads have strongly Bootstrap support.

 Asteraceae, is a cosmopolitan family in terms of natural chemistry, flowering morphology, and adaptation to habitat. Even if there are numerous taxonomic and new molecular phylogenetic studies, the ancestors of the Asteraceae family are not well defined. Study material under consideration, Carlina L., is a member of the tribe Cardueae which belong to Cichorioideaea subfamily of the Asteraceae. The genus Carlina is represented by C. lanata, C. vulgaris, C. tragacanthifolia, C. biebersteinii, C. intermedia, C. oligocephala, C. involucrata subsp. libanotica, C. corymbosa, C.graeca in The Flora of Turkey. In this study, molecular phylogenetic analysis of the genus Carlina, which has a natural distribution in Turkey, has been made. The DNA isolation was performed using phenol- chloroform- isoamylalcohol. ITS Internal Transcribed Region of the nuclear ribosomal DNA nrDNA and the trnL-F Transfer RNA coding region of the chloroplast DNA sequences were used as molecular markers. Carlina was selected as ingroup taxon and Cardopatium, Atractylis and Carthamus were outgroup taxa. Taxa belonging to the genus Carlina distributed in Turkey were analyzed phylogenetically using Branch-and-Bound algorithm with maximum parsimony criterion. Data set analysis using 952 best parsimony trees was made. One tree topology was the most reliable and clads have strongly Bootstrap support.

 Asteraceae, is a cosmopolitan family in terms of natural chemistry, flowering morphology, and adaptation to habitat. Even if there are numerous taxonomic and new molecular phylogenetic studies, the ancestors of the Asteraceae family are not well defined. Study material under consideration, Carlina L., is a member of the tribe Cardueae which belong to Cichorioideaea subfamily of the Asteraceae. The genus Carlina is represented by C. lanata, C. vulgaris, C. tragacanthifolia, C. biebersteinii, C. intermedia, C. oligocephala, C. involucrata subsp. libanotica, C. corymbosa, C.graeca in The Flora of Turkey. In this study, molecular phylogenetic analysis of the genus Carlina, which has a natural distribution in Turkey, has been made. The DNA isolation was performed using phenol- chloroform- isoamylalcohol. ITS Internal Transcribed Region of the nuclear ribosomal DNA nrDNA and the trnL-F Transfer RNA coding region of the chloroplast DNA sequences were used as molecular markers. Carlina was selected as ingroup taxon and Cardopatium, Atractylis and Carthamus were outgroup taxa. Taxa belonging to the genus Carlina distributed in Turkey were analyzed phylogenetically using Branch-and-Bound algorithm with maximum parsimony criterion. Data set analysis using 952 best parsimony trees was made. One tree topology was the most reliable and clads have strongly Bootstrap support.

 Asteraceae, doğal kimyası, çiçeklenme morfolojisi ve habitata uyumu açısından kozmopolit bir familyadır. Çok sayıda taksonomik ve yeni moleküler filogenetik çalışma olsa da, Asteraceae familyasının ataları tam olarak tanımlanmamıştır. İncelenen çalışma materyali, Carlina L., Asteraceaenin Cichorioideaea alt familyasına ait Cardueae kabilesinin bir üyesidir. Carlina cinsi, Türkiye Florasında C. lanata, C. vulgaris, C. tragacanthifolia, C. biebersteinii, C. intermedia, C. oligocephala, C. involucrata subsp. libanotica, C. corymbosa, C.graeca ile temsil edilir. Bu çalışmada, Türkiyede doğal yayılış gösteren Carlina cinsinin moleküler filogenetik analizi yapılmıştır. DNA izolasyonu fenol-kloroform-izoamilalkol kullanılarak yapıldı. Moleküler markör olarak nükleer ribozomal DNAnın nrDNA ITS Internal Transkripsiyonlu Bölge ve kloroplast DNA sekanslarının trnL-F Transfer RNA kodlama bölgesi kullanıldı. İç grup taksonu olarak Carlina, dış grup taksonu olarak Cardopatium, Atractylis ve Carthamus seçilmiştir. Türkiyede yayılış gösteren Carlina cinsine ait taksonlar, maksimum parsimoni kriteri ile Branch-and-Bound algoritması kullanılarak filogenetik olarak analiz edilmiştir. 952 en iyi parsimoni ağacı kullanılarak veri seti analizi yapılmıştır. Bir ağaç topolojisi en güvenirlikte ve dallar güçlü bir şekilde Bootstrap desteğine sahiptir.

Article Details

Molecular phylogenetic analysis of the taxa belonging to the genus Carlina L.(Asteraceae) in Turkey

Authors:

Berna Sanön

Publisher:

Balıkesir University

Main Topics:

Bitki Morfolojisi ve Anatomisi

Journal:

Balıkesir Üniversitesi Fen Bilimleri Enstitüsü Dergisi Vol.26 Issue 1, pp.249-259

Document Type:

Article

Publication Date:

1/2024

Document Language:

English

Document ID (Org No.):

2001303636

pISSN:

1301-7985

asteraceae

cardueae

ITS

trnLF

Phylogenetic analysisAsteraceae

Cardueae

ITS

trnL-F

Filogenetik Analiz

Issues

Article Details

Authors: Berna Sanön

Publisher: Balıkesir University

Main Topics: Bitki Morfolojisi ve Anatomisi

Journal: Balıkesir Üniversitesi Fen Bilimleri Enstitüsü Dergisi Vol.26 Issue 1, pp.249-259

Document Type: Article

Publication Date: 1/2024

Document Language: English

Document ID (Org No.): 2001303636

pISSN: 1301-7985

Issues

Authors:

Berna Sanön

Publisher:

Balıkesir University

Main Topics:

Bitki Morfolojisi ve Anatomisi

Journal:

Balıkesir Üniversitesi Fen Bilimleri Enstitüsü Dergisi Vol.26 Issue 1, pp.249-259

Document Type:

Article

Publication Date:

1/2024

Document Language:

English

Document ID (Org No.):

2001303636

pISSN:

1301-7985