In vitro and in vivo characterization of charcoal rot disease (Macrophomina phaseolina) in sesame

Mehmet Aydoğdu; İlker Kurbetli

Article Details

In vitro and in vivo characterization of charcoal rot disease (Macrophomina phaseolina) in sesame

Authors:

Mehmet Aydoğdu | İlker Kurbetli

Publisher:

Ege University

Main Topics:

Fitopatoloji

Journal:

Ege Üniversitesi Ziraat Fakültesi Dergisi Vol.61 Issue 2, pp.151-163

Document Type:

Article

Publication Date:

1/2024

Document Language:

English

Document ID (Org No.):

2001322037

pISSN:

1018-8851

Keywords:

Abstract:

 Objective: This study aimed to characterize charcoal rot pathogen Macrophomina phaseolina of sesame in vitro and in vivo. Material and Methods: In 2017, two isolates of M. phaseolina were isolated from symptomatic sesame plants in Aksu district of the Western Mediterranean region of Türkiye. Pathogenicity tests were conducted using cv. Muganlı-57 in a greenhouse. Micromorphological characteristics colony growth, colony color, mycelium, and microsclerotium and chlorate sensitivity of the isolates were determined in vitro. Effects of temperature on colony growth and symptoms of charcoal rot in the field were also examined in detail. Results: Significant P0.01 differences were detected in microsclerotium length, microsclerotium width, and colony growths of the isolates on minimal medium. Both isolates were characterized as chlorate-sensitive. On minimal medium, Aksus1 isolate displayed a feathery growth pattern, while Aksus2 isolate showed a restricted growth pattern. Temperature significantly P0.01 affected colony growth. The highest colony growths were detected at 35C, while the lowest ones were found at 40C. Conclusion: The results may provide new insights into the epidemiology of the charcoal rot disease. To the best of our knowledge, this is the first detailed characterization of microscopic traits and charcoal rot symptoms on sesame in the field in the Western Mediterranean region of Türkiye.

 Objective: This study aimed to characterize charcoal rot pathogen Macrophomina phaseolina of sesame in vitro and in vivo. Material and Methods: In 2017, two isolates of M. phaseolina were isolated from symptomatic sesame plants in Aksu district of the Western Mediterranean region of Türkiye. Pathogenicity tests were conducted using cv. Muganlı-57 in a greenhouse. Micromorphological characteristics colony growth, colony color, mycelium, and microsclerotium and chlorate sensitivity of the isolates were determined in vitro. Effects of temperature on colony growth and symptoms of charcoal rot in the field were also examined in detail. Results: Significant P0.01 differences were detected in microsclerotium length, microsclerotium width, and colony growths of the isolates on minimal medium. Both isolates were characterized as chlorate-sensitive. On minimal medium, Aksus1 isolate displayed a feathery growth pattern, while Aksus2 isolate showed a restricted growth pattern. Temperature significantly P0.01 affected colony growth. The highest colony growths were detected at 35C, while the lowest ones were found at 40C. Conclusion: The results may provide new insights into the epidemiology of the charcoal rot disease. To the best of our knowledge, this is the first detailed characterization of microscopic traits and charcoal rot symptoms on sesame in the field in the Western Mediterranean region of Türkiye.

 Objective: This study aimed to characterize charcoal rot pathogen Macrophomina phaseolina of sesame in vitro and in vivo. Material and Methods: In 2017, two isolates of M. phaseolina were isolated from symptomatic sesame plants in Aksu district of the Western Mediterranean region of Türkiye. Pathogenicity tests were conducted using cv. Muganlı-57 in a greenhouse. Micromorphological characteristics colony growth, colony color, mycelium, and microsclerotium and chlorate sensitivity of the isolates were determined in vitro. Effects of temperature on colony growth and symptoms of charcoal rot in the field were also examined in detail. Results: Significant P0.01 differences were detected in microsclerotium length, microsclerotium width, and colony growths of the isolates on minimal medium. Both isolates were characterized as chlorate-sensitive. On minimal medium, Aksus1 isolate displayed a feathery growth pattern, while Aksus2 isolate showed a restricted growth pattern. Temperature significantly P0.01 affected colony growth. The highest colony growths were detected at 35C, while the lowest ones were found at 40C. Conclusion: The results may provide new insights into the epidemiology of the charcoal rot disease. To the best of our knowledge, this is the first detailed characterization of microscopic traits and charcoal rot symptoms on sesame in the field in the Western Mediterranean region of Türkiye.

 Amaç: Bu çalışma susam bitkisinde kömür çürüklüğü patojeninin Macrophomina phaseolina laboratuvar ve tarla şartlarında karakterize edilmesini amaçlamıştır. Materyal ve Yöntem: 2017 yılında, M. phaseolinanın iki izolatı Batı Akdeniz Bölgesinde Aksu lokasyonunda hastalık belirtisi gösteren bitkilerden izole edilmiştir. Patojenisite testleri serada Muganlı-57 çeşidi kullanılarak yapılmıştır. İzolatların mikromorfolojik özellikleri koloni gelişmesi, koloni rengi, miselyum ve mikrosklerot ve klorat hassasiyetleri laboratuvar koşullarında belirlenmiştir. Sıcaklığın koloni gelişmesi üzerine etkisi ve tarlada kömür çürüklüğü hastalığının belirtileri ayrıca detaylı olarak incelenmiştir. Araştırma Bulguları: Minimal ortamda izolatların mikrosklerot uzunluğu, mikrosklerot genişliği ve koloni gelişmelerinde önemli 1 farklılıklar tespit edilmiştir. Her iki izolat klorata hassas olarak karakterize edilmiştir. Minimal ortamda Aksus1 izolatı tüylü bir gelişme deseni gösterirken, Aksus2 izolatı kısıtlanmış bir gelişme deseni göstermiştir. Sıcaklık izolatların koloni gelişmelerini önemli 1 derecede etkilemiştir. En yüksek koloni gelişmeleri 35C de tespit edilirken, en düşük gelişmeler 40C de bulunmuştur. Sonuç: Sonuçlar kömür çürüklüğü hastalığının epidemiyolojisinin anlaşılmasına yeni katkılar sunmaktadır. Bildiğimiz kadarıyla, bu Türkiyenin Batı Akdeniz Bölgesinde susam bitkilerinde kömür çürüklüğü hastalığının mikroskobik özelliklerini ve tarlada belirtilerini detaylı olarak karakterize eden ilk çalışmadır.

Article Details

In vitro and in vivo characterization of charcoal rot disease (Macrophomina phaseolina) in sesame

Authors:

Mehmet Aydoğdu | İlker Kurbetli

Publisher:

Ege University

Main Topics:

Fitopatoloji

Journal:

Ege Üniversitesi Ziraat Fakültesi Dergisi Vol.61 Issue 2, pp.151-163

Document Type:

Article

Publication Date:

1/2024

Document Language:

English

Document ID (Org No.):

2001322037

pISSN:

1018-8851

Field symptoms

fungal disease

fungal traits

Sesamum indicumTarla belirtileri

fungal hastalık

fungal özellikler

Sesamum indicum

Issues

Article Details

Authors: Mehmet Aydoğdu | İlker Kurbetli

Publisher: Ege University

Main Topics: Fitopatoloji

Journal: Ege Üniversitesi Ziraat Fakültesi Dergisi Vol.61 Issue 2, pp.151-163

Document Type: Article

Publication Date: 1/2024

Document Language: English

Document ID (Org No.): 2001322037

pISSN: 1018-8851

Issues

Authors:

Mehmet Aydoğdu | İlker Kurbetli

Publisher:

Ege University

Main Topics:

Fitopatoloji

Journal:

Ege Üniversitesi Ziraat Fakültesi Dergisi Vol.61 Issue 2, pp.151-163

Document Type:

Article

Publication Date:

1/2024

Document Language:

English

Document ID (Org No.):

2001322037

pISSN:

1018-8851